Mechanical loading and TGF-beta regulate proteoglycan synthesis in tendon

Archives of Biochemistry and Biophysics
J R RobbinsK G Vogel

Abstract

Fibrocartilage is found in tendon at sites where the tissue is subjected to transverse compressive loading in vivo. A significant characteristic of the tissue transition from tendon to fibrocartilage in bovine deep flexor tendon is increased gene expression, synthesis, and accumulation of both a large proteoglycan, aggrecan, and a small proteoglyoan, biglycan. In order to investigate the cellular events involved in this response, segments of fetal bovine deep flexor tendon were subjected in vitro to cyclic compressive load for 72 h. Following loading, the level of aggrecan mRNA in cells from loaded tissue was increased 200-450% compared to matched nonloaded tissue segments, as determined by slot-blot analysis. The level of biglycan mRNA increased 100%, and the level of versican mRNA increased 130% in the loaded tissue. The level of decorin mRNA remained virtually unchanged, while expression of alpha 1(I) collagen increased only 40%. When tissue segments were cultured in the presence of transforming growth factor (TGF)-beta 1 (1 ng/ml), the synthesis and expression of mRNA for both aggrecan and biglycan increased, whereas decorin expression was not affected. Similarity in both the direction and the pattern of the cellular respon...Continue Reading

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