Mechanical milling and membrane separation for increased ethanol production during simultaneous saccharification and co-fermentation of rice straw by xylose-fermenting Saccharomyces cerevisiae

Bioresource Technology
Kengo SasakiAkihiko Kondo

Abstract

Mechanical milling and membrane separation were applied to simultaneous saccharification and co-fermentation from hydrothermally pretreated rice straw. Mechanical milling with minimized 4 cycles enabled 37.5±3.4gL(-1) and 45.3±4.4gL(-1) of ethanol production after 48h by xylose-fermenting Saccharomyces cerevisiae from solid fractions (200 and 250gL(-1)) of pretreated rice straw with 5 filter paper unitg-biomass(-1) cellulase (respectively, 77.3±7.1% and 74.7±7.3% of theoretical ethanol yield). Use of a membrane-based process including nanofiltration and ultrafiltration increased the sugar concentrations in the liquid fraction of pretreated rice straw and addition of this liquid fraction to 250gL(-1) solid fraction increased ethanol production to 52.0±0.4gL(-1) (73.8±0.6% of theoretical ethanol yield). Mechanical milling was effective in increasing enzymatic hydrolysis of the solid fraction and membrane separation steps increased the ethanol titer during co-fermentation, leading to a proposal for combining these processes for ethanol production from whole rice straw.

References

Mar 9, 2006·Current Opinion in Chemical Biology·Kevin A GrayMark Emptage
Oct 20, 2006·Trends in Biotechnology·B Hahn-HägerdalG Zacchi
Feb 9, 2008·Nature Biotechnology·Lee R LyndCharles E Wyman
Feb 7, 2009·Bioresource Technology·Akihiro HidenoShigeki Sawayama
Dec 1, 2009·Bioresource Technology·Parameswaran BinodAshok Pandey
Dec 8, 2009·Bioresource Technology·Ralph E H SimsMichael Taylor
Dec 17, 2009·Bioresource Technology·W E Mabee, J N Saddler
Dec 25, 2009·Bioresource Technology·Farid TalebniaIrini Angelidaki
Dec 20, 2011·Current Opinion in Biotechnology·Daniel G OlsonLee R Lynd
Feb 7, 2012·Biotechnology Advances·Yi HeBao-Qiang Liao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.