Mechanical properties of biomimetic tissue adhesive based on the microbial transglutaminase-catalyzed crosslinking of gelatin

Biomacromolecules
Martin K McDermottGregory F Payne

Abstract

Fibrin sealants are a type of soft tissue adhesive that employs biochemical reactions from the late stages of the blood coagulation cascade. Intrinsic to these adhesives are a structural protein and a transglutaminase crosslinking enzyme. We are investigating an alternative biomimetic adhesive based on gelatin and a calcium-independent microbial transglutaminase (mTG). Rheological measurements show that mTG catalyzes the conversion of gelatin solutions into hydrogels, and gel times are on the order of minutes depending on the gelatin type and concentration. Tensile static and dynamic loading of the adhesive hydrogels in bulk form demonstrated that the Young's modulus ranged from 15 to 120 kPa, and these bulk properties were comparable to those reported for hydrogels obtained from fibrin-based sealants. Lap-shear adhesion tests of porcine tissue were performed using a newly published American Society for Testing and Materials (ASTM) standard for tissue adhesives. The gelatin-mTG adhesive bound the opposing tissues together with ultimate adhesive strengths of 12-23 kPa which were significantly higher than the strength observed for fibrin sealants. Even after failure, strands of the gelatin-mTG adhesive remained attached to both o...Continue Reading

References

Feb 1, 1990·Trends in Biotechnology·R L Strausberg, R P Link
Dec 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C S GreenbergR H Rice
Apr 1, 1990·International Journal of Biological Macromolecules·J H Waite
Jun 1, 1994·The Annals of Thoracic Surgery·I C EnnkerR Hetzer
Jun 1, 1996·Journal of Biomedical Materials Research·Y OtaniY Ikada
Sep 1, 1996·Biomaterials·K S AnsethL Brannon-Peppas
Oct 30, 1998·Biomaterials·M DonkerwolckeD Muster
Jan 20, 1999·Bioconjugate Chemistry·J C Schense, J A Hubbell
Jul 1, 1992·Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials·D H SierraS Huang
Jul 1, 1990·Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials·Y C TsengS Hitomi
Jul 9, 1999·Journal of Biomedical Materials Research·S MatsudaY Ikada
Jul 10, 1999·The Journal of Biological Chemistry·L M McDowellJ Schaefer
Jul 27, 1999·Journal of Biomedical Materials Research·Y Nakayama, T Matsuda
Dec 14, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S E SakiyamaJ A Hubbell
Mar 4, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·S E Sakiyama-Elbert, J A Hubbell
Mar 4, 2000·The Journal of Biological Chemistry·J C Schense, J A Hubbell
Sep 22, 2000·Biochemistry·L A Burzio, J H Waite
Sep 28, 2000·Journal of Biomedical Materials Research·D H SierraJ E Lemons
Oct 6, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·S E Sakiyama-Elbert, J A Hubbell
Oct 20, 2000·Retina·E MargalitM S Humayun
Jun 8, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·A H ZischJ A Hubbell
Aug 16, 2001·Journal of Biomedical Materials Research·D G WallaceG C Coker
Sep 22, 2001·American Journal of Surgery·M R Jackson
Sep 22, 2001·American Journal of Surgery·W D Spotnitz
Dec 18, 2001·Journal of Biomedical Materials Research·David H SierraJack E Lemons
Jan 17, 2003·Biotechnology and Bioengineering·Reynaldo VillalongaRaffaele Porta
Jul 16, 2003·Cellular and Molecular Life Sciences : CMLS·R Adány, H Bárdos
Jul 17, 2003·Bioconjugate Chemistry·Joel H Collier, Phillip B Messersmith

❮ Previous
Next ❯

Citations

Mar 23, 2011·Biomacromolecules·Abigail M OelkerMark W Grinstaff
Mar 3, 2006·Lab on a Chip·A Paguirigan, D J Beebe
May 28, 2013·Tissue Engineering. Part B, Reviews·Nihal E VranaAli Khademhosseini
Apr 16, 2014·Biomaterials·Megan L McCainKevin Kit Parker
Jan 30, 2016·ACS Applied Materials & Interfaces·Sevgi BalciogluBurhan Ates
Jan 7, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Chiho Kataoka-HamaiTetsushi Taguchi
Jan 23, 2016·Chemical Reviews·Shin-ichiro ShodaShiro Kobayashi
Aug 3, 2010·Biomaterials·Christopher M ElvinJerome A Werkmeister
May 4, 2010·Colloids and Surfaces. B, Biointerfaces·Fei SongNan-Nan Li
Jan 20, 2010·Acta Biomaterialia·K P Lim, L P Tan
Aug 12, 2009·Journal of Colloid and Interface Science·Qunwei TangJianming Lin
Aug 19, 2008·Trends in Biotechnology·Yang Zhu, Johannes Tramper
Oct 18, 2015·Acta Biomaterialia·Smita GhoshStephen C Moratti
Jun 15, 2007·Biomaterials·Martin EhrbarMatthias P Lutolf
Feb 13, 2007·Current Opinion in Chemical Biology·Gregory F Payne
Sep 17, 2015·Drug Delivery and Translational Research·Christian J TormosSundararajan V Madihally
Apr 16, 2013·Biochimica Et Biophysica Acta·S Thirupathi Kumara RajaA Gnanamani
Apr 11, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Yi LiuGregory F Payne
Nov 10, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Tianhong ChenGregory F Payne
Jun 23, 2007·Journal of Biomedical Materials Research. Part a·C W YungT A Barbari
Feb 9, 2012·Macromolecular Bioscience·Benjamin F PierceAndreas Lendlein
Feb 11, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Muzhou Wang, Julia A Kornfield
Mar 27, 2012·Journal of Biomedical Materials Research. Part a·Selestina Gorgieva, Vanja Kokol
May 17, 2014·Advanced Healthcare Materials·Huaping TanJingxuan Yan
Mar 11, 2010·Advanced Materials·Peter DomachukDavid L Kaplan
Jun 17, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Nasim AnnabiAli Khademhosseini
Dec 23, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Michael BlatchleySharon Gerecht
Mar 29, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Brittany L TaylorJoseph W Freeman
Apr 22, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Francesca ScognamiglioSergio Paoletti
Mar 15, 2016·Journal of Materials Science. Materials in Medicine·A I BochyńskaP Buma
Feb 5, 2015·Chemical Society Reviews·C Ghobril, M W Grinstaff
Jan 24, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Ai UshiyamaYoshihisa Kaizuka
May 6, 2016·Scientific Reports·Clement MarmoratMiriam Rafailovich
Jun 29, 2016·Scientific Reports·Archana BettadapurMegan L McCain
Oct 31, 2017·Biofabrication·Kevin D Roehm, Sundararajan V Madihally
Sep 3, 2010·Biofabrication·Yi LiuGregory F Payne
Jan 23, 2020·Materials·Giorgio MatteiAnd Arti Ahluwalia

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Related Papers

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Tianhong ChenGregory F Payne
Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials
D H SierraS Huang
© 2022 Meta ULC. All rights reserved