Mechanical stimulation increases expression of acetylcholinesterase in cultured myotubes

The American Journal of Physiology
D A Hubatsch, B J Jasmin

Abstract

We tested the hypothesis that acetylcholinesterase (AChE) expression in skeletal muscle cells is increased by passive mechanical stimulation. To this end, primary cultures of myotubes were subjected to repeated cycles of stretch-relaxation for 5 min, 30 min, 3 h, and 24 h, using the Flexercell FX-2000 strain unit. Although mechanical stimulation did not affect AChE expression at early time points, it led to a significant increase (42%; P < 0.05) in total AChE activity at 24 h. This increase reflected a general elevation in the activity of all AChE molecular forms as opposed to a preferential increase in a specific form. Tetrodotoxin (TTX) treatment did not prevent the increase in AChE expression, whereas nifedipine partially blocked it. These changes in enzyme expression were accompanied by increases in the levels of AChE mRNA, suggesting the involvement of pretranslational regulatory mechanisms. Together, these results illustrate that, in addition to neural activation and trophic factors, passive mechanical forces modulate expression of AChE in skeletal muscle cells. Because TTX did not prevent the increase in AChE expression, it appears that the effects of mechanical stimulation are independent of electrical activity, which f...Continue Reading

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Citations

Jan 25, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·C Boudreau-LarivièreB J Jasmin
Jun 19, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·W MatarJ M Renaud
Sep 5, 2002·Muscle & Nerve·Erica A WehrweinJohn M Spitsbergen
Sep 18, 2009·The Journal of Biological Chemistry·Carlos A Ruiz, Richard L Rotundo
Apr 9, 2011·Biotechnology Letters·S PasseyM P Lewis
Jun 11, 2009·The Journal of Biological Chemistry·Carlos A Ruiz, Richard L Rotundo
Oct 1, 2011·International Journal of Toxicology·Michael W PerkinsMadhusoodana P Nambiar
Dec 26, 2001·Journal of Neuroscience Research·Peter Pregelj, Janez Sketelj
Jun 22, 2006·Journal of Neurochemistry·Tatiana V Cohen, William R Randall
Jan 23, 2016·Journal of Tissue Engineering·Hye-Sun YuIvan Wall
Mar 16, 2016·Journal of Tissue Engineering·Hye-Sun YuIvan Wall
Mar 11, 1999·Current Opinion in Neurobiology·S M Hughes, P C Salinas
Aug 20, 2021·Frontiers in Physiology·Dapeng RenXiao Yuan

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