Mechanical strain effect on bone-resorbing activity and messenger RNA expressions of marker enzymes in isolated osteoclast culture

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
K KurataY Iwamoto

Abstract

Adaptive modeling and remodeling are controlled by the activities of osteoblasts and osteoclasts, which are capable of sensing their mechanical environments and regulating deposition or resorption of bone matrix. The effects of mechanical stimuli on isolated osteoclasts have been scarcely examined because it has proven to be difficult to prepare a number of pure osteoclasts and to cultivate them on mineralized substratum during mechanical stimulation. Recently, we developed an apparatus for applying mechanical stretching to the ivory slice/plastic plate component on which cells could be cultured. The loading frequency, strain rate, and generated strain over an ivory surface could be controlled by a personal computer. Using this apparatus, we examined the role of mechanical stretching on the bone-resorbing activity of the osteoclasts. Mature and highly enriched osteoclasts were cultured for 2, 12, and 24 h on the ivory/plate component while being subjected to intermittent tensile strain. The stretched osteoclasts showed enhanced messenger RNA (mRNA) expression levels of osteoclast marker enzymes, tartrate-resistant acid phosphatase (TRAP), and cathepsin K and increases of resorbed-pit formation, suggesting that the mechanical st...Continue Reading

References

May 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C T BrightonJ Williamson
Mar 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H M Frost
May 1, 1991·Journal of Biomechanical Engineering·S C CowinM L Moss
Nov 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C T BrightonC C Clark
Jan 1, 1987·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C T Rubin, L E Lanyon
Sep 1, 1987·The Anatomical Record·H M Frost
Mar 1, 1972·Journal of Biomechanics·A Chamay, P Tschantz
May 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R A Hillam, T M Skerry
Mar 1, 1995·Pflügers Archiv : European journal of physiology·A WiltinkB Van Duijn
Jan 1, 1993·Calcified Tissue International·L E Lanyon
Feb 1, 1993·Bone and Mineral·T J ChambersJ W Chow
May 1, 1996·Endocrinology·Y Mikuni-TakagakiS Saito
Feb 1, 1997·Journal of Cellular Physiology·J Klein-NulendE H Burger
Mar 28, 1997·The Journal of Biological Chemistry·T InuiT Yamamura
May 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G ZamanL E Lanyon
Jun 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T SudaN Takahashi
Jul 1, 1997·The Journal of Bone and Joint Surgery. American Volume·W D BugbeeC A Engh
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·H YasudaT Suda
May 16, 1998·The Journal of Biological Chemistry·E JimiT Suda
May 8, 1998·Biochemical and Biophysical Research Communications·T KamedaM Kumegawa
Jun 11, 1998·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M C MeazziniL C Gerstenfeld
Aug 25, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·F HaraS Tanaka

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Citations

Oct 30, 2014·Current Opinion in Endocrinology, Diabetes, and Obesity·William R ThompsonJanet Rubin
Dec 3, 2014·Journal of Biomechanics·Yousef ShafieyanThomas M Quinn
Apr 27, 2007·PloS One·Philipp LeuchtJill A Helms
Apr 26, 2012·Annals of Biomedical Engineering·Ping LiDing Zhang
Jul 3, 2015·Experimental and Therapeutic Medicine·Fengbo LiXinlong Ma
Sep 2, 2014·Advanced Drug Delivery Reviews·Maureen E Lynch, Claudia Fischbach
Jul 13, 2006·Annals of the New York Academy of Sciences·Bruce R Troen
Apr 25, 2006·Experimental Dermatology·Shoichiro YanoKunihiko Tamaki
Feb 27, 2007·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Hisashi IchimiyaTatsuji Nishihara
Aug 3, 2017·International Journal of Molecular Sciences·Zhihao ChenAirong Qian
Jun 20, 2002·Journal of Biomechanical Engineering·Jiro NagatomiRena Bizios
Jun 15, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Michael J GardnerMathias P Bostrom
Feb 12, 2008·Journal of Cellular Physiology·Fumitaka MizoguchiMasaki Noda
Dec 10, 2008·Chembiochem : a European Journal of Chemical Biology·Dafna GeblingerLia Addadi
Dec 21, 2005·Journal of Cellular Biochemistry·Monica MoniciPietro Antonio Bernabei
Dec 17, 2014·Cellular and Molecular Life Sciences : CMLS·Frances Y McWhorterWendy F Liu
Jan 8, 2013·International Journal of Molecular Medicine·Sumika KameyamaJunichiro Iida
Sep 14, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Irena LavelinMark Pines
Jul 10, 2007·Journal of Biomedical Materials Research. Part a·Yasutaka YamadaRacquel Z LeGeros
Jun 15, 2013·Clinical Implant Dentistry and Related Research·Chendi XuEryi Lu
Jan 27, 2019·International Journal of Molecular Sciences·Dominique MuschterSusanne Grässel
Jun 16, 2010·Molecular and Cellular Biochemistry·Zhang Qing HongLiu Li
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Qianli HuangYong Liu
Jul 3, 2021·International Journal of Molecular Sciences·Xia XuLifang Hu

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