Mechanical strain increases endothelin-1 gene expression via protein kinase C pathway in human endothelial cells

Journal of Cellular Physiology
D L WangJ J Wang

Abstract

Vascular endothelial cells (ECs) are constantly subjected to mechanical strain due to relaxation and contraction of vessel walls. The effects of cyclical strain on endothelin-1 (Et-1) secretion and Et-1 mRNA levels in human umbilical vein ECs were examined. Cultured ECs grown on a flexible membrane base were deformed by negative pressure (16 kPa at 60 cycles/min). Cells subjected to strain showed increased Et-1 secretion (0.54 ng/hr/10(6) cells) compared with unstrained control cells (0.22 ng/hr/10(6) cells). Northern blot analysis of cells strained for 2 hours or longer demonstrated a sustained elevated Et-1 mRNA level at more than double the level in unstrained controls. This strain-induced ET-1 mRNA level returned to its basal level 2 hours after the release of strain. Cells treated with actinomycin D before or during strain treatment showed no strain-induced gene expression. Pretreatment of ECs with a protein kinase C (PKC) inhibitor, Calphostin C, strongly inhibited the strain-induced Et-1 gene expression. Pretreatment of ECs with cAMP- or cGMP-dependent protein kinase inhibitors (KT5720 or KT5823) only partially inhibited the increased Et-1 mRNA levels in strain-treated cells. EGTA strongly inhibited the Et-1 gene express...Continue Reading

References

May 1, 1977·Atherosclerosis·K N BrethertonS L Skinner
Oct 13, 1992·Annals of the New York Academy of Sciences·M U NollertL V McIntire
Apr 1, 1992·Journal of Cellular Physiology·J A CarosiL V McIntire
Apr 1, 1992·The American Journal of Physiology·O R Rosales, B E Sumpio
Mar 31, 1992·Biochemical and Biophysical Research Communications·D L WangC C Liew
Feb 1, 1992·Hypertension·T F LüscherZ H Yang
Oct 13, 1991·The New England Journal of Medicine·A LermanJ C Burnett
Sep 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G M Rubanyi, L H Botelho
May 1, 1990·Hypertension·M ShichiriF Marumo
Jun 1, 1990·The American Journal of Medicine·M KohnoT Takeda
Jan 18, 1990·The New England Journal of Medicine·C J Murray
Jan 1, 1990·Journal of Biomechanics·M J ThubrikarR T Eppink
Jun 15, 1989·Biochemical and Biophysical Research Communications·P Cernacek, D J Stewart
Nov 1, 1983·Progress in Cardiovascular Diseases·A V Chobanian
Sep 15, 1993·Biochemical and Biophysical Research Communications·D L WangJ J Wang
Jan 1, 1993·Journal of Cellular Physiology·H J HsiehJ A Frangos
Mar 31, 1993·Biochemical and Biophysical Research Communications·J A WinklesP Libby
Jun 17, 1993·The New England Journal of Medicine·A GiaidD J Stewart
Jul 1, 1992·Trends in Cardiovascular Medicine·R J HilkertT Quertermous

❮ Previous
Next ❯

Citations

Nov 26, 1999·Journal of Cellular Biochemistry·M T Rizzo, W m Yu
Aug 6, 2011·Cell Biochemistry and Biophysics·Peter K StricklettDonald E Kohan
Dec 25, 2003·The Proceedings of the Nutrition Society·Tracy C Grikscheit
Sep 15, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lisa R StowBrian D Cain
Jul 20, 2005·Clinical and Experimental Pharmacology & Physiology·Wei-Fung BiNen-Chung Chang
Apr 24, 2008·Clinical and Experimental Pharmacology & Physiology·Chi-Sheng ChiouPaul Chan
Jan 24, 2003·American Journal of Respiratory Cell and Molecular Biology·Daniel J TschumperlinJeffrey M Drazen
Sep 15, 1998·Kidney International. Supplement·B J BallermannA Liu
Feb 24, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Carolyn HolladayAbhay Pandit
Dec 30, 2014·Particle and Fibre Toxicology·Christian FreeseC James Kirkpatrick
May 26, 2005·Brain Research. Brain Research Reviews·Lyle W Ostrow, Frederick Sachs
Jan 17, 2017·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Marzena Wojewodzka-ZelezniakowiczEwa Chabielska
Jul 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Ernesto L Schiffrin, Rhian M Touyz
Nov 19, 2003·Hypertension·Ju-Chi LiuTzu-Hurng Cheng
Oct 9, 2002·The Journal of Biological Chemistry·Jing-Jy ChengDanny Ling Wang
Apr 27, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M CattaruzzaM Hecker
May 8, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Shu-Hui JuanTzu-Hurng Cheng
Jul 18, 2001·The Journal of Biological Chemistry·M CattaruzzaM Hecker
Jun 3, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·B ResslerR D Kamm
May 18, 2001·American Journal of Physiology. Heart and Circulatory Physiology·J P LoennechenO Ellingsen
Feb 5, 2002·Endothelium : Journal of Endothelial Cell Research·D S WangP S Tsao
Jan 1, 1997·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·D E Kohan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiovascular Homeostasis

Cardiovascular regulation and homeostasis is important in maintaining health and dysfunction may lead to cardiovascular diseases. Nitric oxide and vascular endothelium are one of many molecules involved in the homeostatic mechanism. Here is the latest research on cardiovascular homeostasis.