Mechanical stress regulates bone regulatory gene expression independent of estrogen and vitamin D deficiency in rats.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Ashwini Kumar NepalN Bravenboer

Abstract

Mechanical stress determines bone mass and structure. It is not known whether mechanical loading affects expression of bone regulatory genes in a combined deficiency of estrogen and vitamin D. We studied the effect of mechanical loading on the messenger RNA (mRNA) expression of bone regulatory genes during vitamin D and/or estrogen deficiency. We performed a single bout in vivo axial loading with 14 N peak load, 2 Hz frequency and 360 cycles in right ulnae of nineteen weeks old female control Wistar rats with or without ovariectomy (OVX), vitamin D deficiency and the combination of OVX and vitamin D deficiency (N = 10/group). Total bone RNA was isolated 6 hours after loading, and mRNA expression was detected of Mepe, Fgf23, Dmp1, Phex, Sost, Col1a1, Cyp27b1, Vdr, and Esr1. Serum levels of 25(OH)D, 1,25(OH)2 D and estradiol were also measured at this time point. The effect of loading, vitamin D and estrogen deficiency and their interaction on bone gene expression was tested using a mixed effect model analysis. Mechanical loading significantly increased the mRNA expression of Mepe, and Sost, whereas it decreased the mRNA expression of Fgf23 and Esr1. Mechanical loading showed a significant interaction with vitamin D deficiency wi...Continue Reading

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Citations

Sep 6, 2020·Journal of Bone and Mineral Metabolism·Alann Thaffarell Portilho SouzaMarcio Mateus Beloti

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Methods Mentioned

BETA
hormone replacement therapy
sedation
Assay
PCR

Software Mentioned

NIS Elements
Light Cycler 480
R

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