Mechanical Tension Promotes Formation of Gastrulation-like Nodes and Patterns Mesoderm Specification in Human Embryonic Stem Cells.

Developmental Cell
Jonathon M MuncieValerie M Weaver

Abstract

Embryogenesis is directed by morphogens that induce differentiation within a defined tissue geometry. Tissue organization is mediated by cell-cell and cell-extracellular matrix (ECM) adhesions and is modulated by cell tension and tissue-level forces. Whether cell tension regulates development by modifying morphogen signaling is less clear. Human embryonic stem cells (hESCs) exhibit an intrinsic capacity for self-organization, which motivates their use as a tractable model of early human embryogenesis. We engineered patterned substrates that recapitulate the biophysical properties of the early embryo and mediate the self-organization of "gastrulation-like" nodes in cultured hESCs. Tissue geometries that generated local nodes of high cell-adhesion tension directed the spatial patterning of the BMP4-dependent "gastrulation-like" phenotype by enhancing phosphorylation and junctional release of β-catenin to promote Wnt signaling and mesoderm specification. Furthermore, direct force application via mechanical stretching promoted BMP-dependent mesoderm specification, confirming that tissue-level forces can directly regulate cell fate specification in early human development.

References

Dec 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·V Hamburger, H L Hamilton
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·S ChengR Higuchi
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·S UrlingerW Hillen
Mar 10, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S C ChapmanA Lumsden
Nov 15, 2002·Developmental Cell·Federica Bertocchini, Claudio D Stern
Aug 5, 2003·Development Genes and Evolution·Jinsong ZhangMichael P Sarras
Mar 3, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Takashi MikawaDavid E Reese
Oct 20, 2004·The Journal of Cell Biology·Cara J Gottardi, Barry M Gumbiner
Jan 26, 2005·Current Biology : CB·Mariann Bienz
May 10, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Caroline KempLuc Leyns
Aug 16, 2005·Current Opinion in Cell Biology·Jack Lilien, Janne Balsamo
Aug 16, 2005·Current Opinion in Cell Biology·Ray Keller
Feb 16, 2008·Annual Review of Biochemistry·Joseph San FilippoHannah Klein
Jul 17, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Lance A DavidsonDouglas W Desimone
Sep 9, 2009·Development·Renée van Amerongen, Roel Nusse
Dec 17, 2009·Cell·Christian P Petersen, Peter W Reddien
Mar 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kristopher A KilianMilan Mrksich
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Qingzong TsengManuel Théry
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Jan 5, 2013·Science·Le CongFeng Zhang
Apr 5, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ashok RamasubramanianShaun S Gordon
Nov 28, 2013·Nature Communications·Thibaut BrunetEmmanuel Farge
May 6, 2014·Current Opinion in Genetics & Development·Larry A Taber
Jun 30, 2014·Nature Methods·Aryeh WarmflashAli H Brivanlou
Mar 22, 2015·Current Opinion in Genetics & Development·Patrick McMillen, Scott A Holley
Aug 4, 2015·The Review of Scientific Instruments·Xiang LiJianping Fu
Aug 11, 2015·Seminars in Cell & Developmental Biology·Hervé Turlier, Jean-Léon Maître
Sep 8, 2015·Methods : a Companion to Methods in Enzymology·L PrzybylaV M Weaver
Oct 18, 2015·Seminars in Cell & Developmental Biology·Hiroshi Hamada
Apr 5, 2016·Nature Materials·Junmin LeeKristopher A Kilian
May 5, 2016·Nature Cell Biology·Marta N ShahbaziMagdalena Zernicka-Goetz
Jul 28, 2016·Cell Stem Cell·Laralynne PrzybylaValerie M Weaver
Mar 4, 2017·Science·Sarah Ellys HarrisonMagdalena Zernicka-Goetz
Mar 16, 2017·Development·Mijo Simunovic, Ali H Brivanlou
Jun 7, 2017·Current Opinion in Cell Biology·Margot Lk Williams, Lilianna Solnica-Krezel
Jan 10, 2018·The Journal of Cell Biology·Charlène GayrardNicolas Borghi

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Citations

Feb 6, 2021·Cell Stem Cell·Jonathon M Muncie, Valerie M Weaver
Mar 6, 2021·Stem Cell Reports·Janet Rossant, Patrick P L Tam
Mar 24, 2021·Developmental Biology·Jake Cornwall-Scoones, Magdalena Zernicka-Goetz
Dec 23, 2020·Developmental Biology·Alexandra Schauer, Carl-Philipp Heisenberg
Apr 24, 2021·Advanced Healthcare Materials·Ian C BergGregory H Underhill
Jun 10, 2021·Developmental Cell·Mary-Kate HaywardValerie M Weaver
Jul 13, 2021·Cells & Development·Nicole Gorfinkiel, Alfonso Martinez Arias
Jul 27, 2021·Trends in Cell Biology·Susanne C van den Brink, Alexander van Oudenaarden
Nov 2, 2021·Cell Cycle·Evangéline Despin-Guitard, Isabelle Migeotte
Nov 3, 2021·Journal of Mathematical Biology·Prajakta BedekarMisha Perepelitsa
Nov 15, 2021·Journal of Molecular Biology·Yunping Chen, Yue Shao

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