MECHANICAL VENTILATION AUGMENTS POLY(I:C)INDUCED LUNG INJURY VIA A WISP1-INTEGRIN β3 DEPENDENT PATHWAY IN MICE

Molecular Medicine
Shuqing JinQuan Li

Abstract

Mechanical ventilation can improve hypoxemia, but can also cause the so-called ventilator-induced lung injury (VILI). Polyinosinic-polycytidylic acid (poly(I:C)), an analogue of natural double strand RNA virus, can induce lung inflammation. The purpose of this study was to determine whether moderate tidal volume mechanical ventilation (MTV) augments Poly(I:C)-induced lung injury, and if so, the mechanism responsible for it. Poly(I:C) (2μg/g) were instilled intratracheally in C57BL/6J wide type (WT) mice. They were then randomized to MTV (10ml/kg tidal volume) or spontaneous breath. Lung tissues and bronchoalveolar lavage fluid (BALF) were collected 4h later for various measurements. Our results showed that MTV did not cause significant injury in normal lungs, but augmented Poly(I:C)-induced lung injury. The expression level of WNT-induced secreted protein 1 (WISP1) was consistent with lung injury, and the amplification of lung injury by MTV can be alleviated by anti-WISP1 antibody treatment. MTV further increased Poly(I:C)-induced integrin β3 expression in the lung. And co-immunoprecipitation (Co-IP) results suggested there was an interaction between WISP1 and β3. WISP1 significantly increased Poly(I:C)-induced TNF-α production...Continue Reading

References

Apr 5, 1996·Science·E C Butcher, L J Picker
Oct 13, 2001·The Journal of Biological Chemistry·L DesnoyersD Pennica
Apr 15, 2003·Toxicology and Applied Pharmacology·Tsung-Ming ShihJohn H McDonough
Mar 23, 2004·Journal of Dermatological Science·Kiyoshi Takeda, Shizuo Akira
Jun 12, 2004·American Journal of Respiratory Cell and Molecular Biology·Quan ShaRobert P Schleimer
Aug 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·William A AltemeierW Conrad Liles
Sep 26, 2006·Critical Care : the Official Journal of the Critical Care Forum·D Shane O'MahonyGustavo Matute-Bello
Jul 14, 2007·American Journal of Respiratory and Critical Care Medicine·Rongqian WuPing Wang
Sep 29, 2007·Translational Research : the Journal of Laboratory and Clinical Medicine·Jaideep MoitraJoe G N Garcia
Mar 11, 2010·Seminars in Immunopathology·Hongyan WangChristopher E Rudd
Aug 5, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Mitsuaki OnoMarian F Young
Mar 15, 2011·The Journal of Biological Chemistry·Rebecca L HeiseStavros Garantziotis
Apr 2, 2011·Biochemical Pharmacology·Chun-Han HouChih-Hsin Tang
Dec 2, 2011·Nature Reviews. Drug Discovery·Joon-Il Jun, Lester F Lau
Mar 29, 2012·Experimental Lung Research·Bin WangSu Min
Jun 16, 2012·American Journal of Respiratory Cell and Molecular Biology·Hui-Hua LiLi-Ming Zhang
Apr 1, 2014·Biochemical and Biophysical Research Communications·Yohei TakedaKunitoshi Imai
Apr 26, 2014·Critical Care : the Official Journal of the Critical Care Forum·Yuda SutherasanPaolo Pelosi

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Apr 14, 2017·Cell Death & Disease·Hsiao-Chi TsaiChih-Hsin Tang
Nov 18, 2018·Critical Care : the Official Journal of the Critical Care Forum·Xibing DingLi-Ming Zhang
Jan 22, 2019·International Journal of Molecular Medicine·Yue-Feng XiaLi-Ming Zhang

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