PMID: 8952950Dec 1, 1996Paper

Mechanically activated currents in chick heart cells

The Journal of Membrane Biology
H Hu, F Sachs

Abstract

As predicted from stretch-induced changes of rate and rhythm in the heart, acutely isolated embryonic chick heart cells exhibit whole-cell mechanosensitive currents. These currents were evoked by pressing on cells with a fire polished micropipette and measured through a perforated patch using a second pipette. The currents were carried by Na+ and K+ but not Cl-, and were independent of external Ca2+. The currents had linear I/V curves reversing at -16 mV and were completely blocked by Gd3+ >/= 30 microM and Grammostola spatulata venom at a dilution of 1:1000. Approximately 20% of cells showed time dependent inactivation. In contrast to direct mechanical stimulation, hypotonic volume stress produced an increase in conductance for anions rather than cations-the two stimuli are not equivalent. The cells had two types of stretch-activated ion channels (SACs): a 21 pS nonspecific cation-selective reversing at -2 mV and a 90 pS K+ selective reversing at -70 mV in normal saline. The activity of SACs was strongly correlated with the presence of whole-cell currents. Both the whole-cell currents and SACs were blocked by Gd3+ and by Grammostola spatulata spider venom. Mechanical stimulation of spontaneously active cells increased the beat...Continue Reading

Citations

Feb 9, 2011·Pflügers Archiv : European journal of physiology·Horacio E CingolaniNéstor G Pérez
Dec 23, 2004·Journal of Molecular Histology·Weihui LiNatalia Trayanova
May 7, 2003·Progress in Biophysics and Molecular Biology·Weizhen Niu, Frederick Sachs
May 7, 2003·Progress in Biophysics and Molecular Biology·Michael R Franz, Frank Bode
Mar 10, 1999·Progress in Biophysics and Molecular Biology·S C Calaghan, E White
Nov 6, 1998·Pharmacology & Therapeutics·A R Wright, S A Rees
Jul 9, 1998·Plastic and Reconstructive Surgery·T TakeiB E Sumpio
Jun 5, 2002·Plastic and Reconstructive Surgery·Roger E De Filippo, Anthony Atala
Oct 24, 1998·Clinical and Experimental Pharmacology & Physiology·D A Saint
Feb 12, 2004·Science's STKE : Signal Transduction Knowledge Environment·Sergei Sukharev, David P Corey
Mar 17, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Yanggan WangBrian H Crawford
Mar 17, 2004·The Journal of Physiology·Tom SchillingClaudia Eder
Feb 11, 1998·The Journal of Physiology·S D Silberberg, K L Magleby
Mar 16, 2000·The Journal of Physiology·Y H ZhangY E Earm
Feb 10, 2016·Differentiation; Research in Biological Diversity·Tim P KelderMonique R M Jongbloed
Mar 21, 2008·Progress in Biophysics and Molecular Biology·Chang Ahn SeolChae Hun Leem
May 13, 2008·Progress in Biophysics and Molecular Biology·Satoshi NishimuraSeiryo Sugiura
Sep 5, 2006·Journal of Cardiovascular Electrophysiology·Frank BodeUwe K H Wiegand
Jan 22, 2003·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Frederick H SilverGurinder P Seehra
Jan 26, 2010·Journal of Molecular and Cellular Cardiology·Yanggan Wang, Joseph A Hill
May 17, 2015·Medical Hypotheses·Tomohisa NagasaoYoshio Tanaka
Jul 31, 2012·Progress in Biophysics and Molecular Biology·Rachel M A ter Bekke, Paul G A Volders
Jul 19, 2002·Biophysical Journal·Guillaume T Charras, Mike A Horton
Dec 25, 2016·Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology·T Alexander Quinn, Peter Kohl
Sep 15, 2000·Cardiology Clinics·P KohlE White
Sep 24, 2004·Hypertension·Dale J Benos
Oct 1, 2008·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Jae Boum YoumYung E Earm
May 10, 2003·Pflügers Archiv : European journal of physiology·Andre KamkinGerrit Isenberg
Mar 29, 2001·Physiological Reviews·O P Hamill, B Martinac
Jul 10, 2001·American Journal of Physiology. Cell Physiology·M J MohantyN F Rossi
Mar 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·R R LambertsN Westerhof
Apr 21, 2001·Cardiovascular Drug Reviews·L C AddingL E Gustafsson
Mar 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·X Wu, M J Davis
Apr 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Peter Linz, Roland Veelken
Feb 26, 2005·Journal of Applied Physiology·Chae-Ryon KongLeslie Tung
Jun 11, 2011·Journal of Applied Physiology·Néstor G PérezHoracio E Cingolani
Feb 9, 2000·American Journal of Physiology. Heart and Circulatory Physiology·T ZengF Sachs
Apr 30, 1999·Physiological Reviews·M J Davis, M A Hill
Jul 14, 2018·Scientific Reports·Hanyu ZhangJack M Rogers

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.