PMID: 8581879Dec 1, 1995Paper

Mechanically induced periosteal bone formation is paralleled by the upregulation of collagen type one mRNA in osteocytes as measured by in situ reverse transcript-polymerase chain reaction

Calcified Tissue International
Y Q SunC T Rubin

Abstract

Reverse transcript polymerase chain reaction (RT-PCR) was developed for use in situ to measure mechanically mediated changes in gene expression activity in osteocytes within dense cortical bone. Using the functionally isolated turkey ulna model of bone adaptation, the left ulna of 6 old adult (36-40 months) male turkeys were subject to 4 weeks of a mechanical regimen consisting either of (1) 3000 microstrain at 1 Hz for 5 minutes/day or (2) 500 microstrain at 30 Hz for 10 minutes/day. The right ulna of each bird remained intact and served as control. Only a small percentage of osteocytes in the intact control bones and the 3000 microstrain ulnae showed any evidence of mRNA for collagen (each 1.2% +/- 0.3%). However, mRNA for collagen type I was strongly evident in 92.4% (+/-2%) of the osteocytes within the ulnae subject to the high frequency, low magnitude load. Sense primer control sections from both experimental and intact animals were used to verify that only osteocytes of the loaded bone had elevated the level of collagen mRNA. This high frequency, low magnitude mechanical stimulus was also sufficient to stimulate substantial new bone formation (14% +/- 5% over intact controls), whereas the low frequency, high magnitude sti...Continue Reading

References

Apr 1, 1992·Calcified Tissue International·C T RubinK J McLeod
Apr 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A van der Plas, P J Nijweide
May 1, 1990·Calcified Tissue International·T M SkerryL E Lanyon
Jan 1, 1989·Journal of Cellular Biochemistry·D A RappoleeZ Werb
Oct 1, 1989·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T M SkerryL E Lanyon
Jan 1, 1987·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C T Rubin, L E Lanyon
Mar 1, 1968·Calcified Tissue Research·C A Bassett
Jan 1, 1981·Calcified Tissue International·S B Doty
May 1, 1994·Annals of Biomedical Engineering·Y ZengS Weinbaum
Jun 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M Z ChengL E Lanyon

❮ Previous
Next ❯

Citations

May 1, 1997·The Journal of Pathology·J H Steel, R Poulsom
Jun 22, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C RubinK McLeod
Nov 1, 1996·Histochemistry and Cell Biology·E M AardenP J Nijweide
May 25, 2007·Current Osteoporosis Reports·Giolanta Kogianni, Brendon S Noble
Jan 8, 1999·Journal of Biomechanics·C R JacobsH J Donahue
Aug 10, 2001·Drug Discovery Today·C T. RubinY -X. Qin
Jun 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T S GrossC T Rubin
May 3, 2014·PloS One·Antonia TorcasioG Harry van Lenthe
Oct 3, 1999·Medical & Biological Engineering & Computing·A J el HajS J Publicover
Jan 14, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ted S GrossSundar Srinivasan
Mar 26, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Kate WardZulf Mughal
May 8, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jelica Gluhak-HeinrichDubravko Pavlin
Oct 19, 2007·Biochemical and Biophysical Research Communications·Kenneth A MyersDavid A Hart
Dec 20, 2005·Gene·Janet RubinChristopher Rae Jacobs
Apr 22, 2008·Archives of Biochemistry and Biophysics·Brendon S Noble
Aug 27, 2005·European Journal of Morphology·Brendon Noble
Jun 22, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C Edward HofflerSteven A Goldstein
May 18, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A H HsiehK L Sung
Jan 26, 1999·Clinical Orthopaedics and Related Research·E Y ChaoH Aro
Sep 14, 1999·The American Journal of Physiology·J S DoddT S Gross

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.