PMID: 12765767May 27, 2003Paper

Mechanics and structure of titin oligomers explored with atomic force microscopy

Biochimica Et Biophysica Acta
Miklós S Z KellermayerH L Granzier

Abstract

Titin is a giant polypeptide that spans half of the striated muscle sarcomere and generates passive force upon stretch. To explore the elastic response and structure of single molecules and oligomers of titin, we carried out molecular force spectroscopy and atomic force microscopy (AFM) on purified full-length skeletal-muscle titin. From the force data, apparent persistence lengths as long as approximately 1.5 nm were obtained for the single, unfolded titin molecule. Furthermore, data suggest that titin molecules may globally associate into oligomers which mechanically behave as independent wormlike chains (WLCs). Consistent with this, AFM of surface-adsorbed titin molecules revealed the presence of oligomers. Although oligomers may form globally via head-to-head association of titin, the constituent molecules otherwise appear independent from each other along their contour. Based on the global association but local independence of titin molecules, we discuss a mechanical model of the sarcomere in which titin molecules with different contour lengths, corresponding to different isoforms, are held in a lattice. The net force response of aligned titin molecules is determined by the persistence length of the tandemly arranged, diff...Continue Reading

References

Sep 11, 1986·Nature·R HorowitsR J Podolsky
Dec 5, 1984·Journal of Molecular Biology·J TrinickA Whiting
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K WangD Palter
Oct 1, 1995·Circulation Research·K TrombitásH Granzier
Sep 9, 1994·Science·C BustamanteS Smith
Feb 19, 1996·FEBS Letters·M S Kellermayer, H L Granzier
Aug 9, 1996·Journal of Molecular Biology·W A LinkeS Labeit
Jan 17, 1997·Journal of Molecular Biology·L Tskhovrebova, J Trinick
Sep 2, 1998·Journal of Structural Biology·M S KellermayerH L Granzier
Feb 27, 1999·Current Opinion in Cell Biology·C C GregorioS Labeit
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M Carrion-VazquezJ M Fernandez
Sep 15, 2000·Advances in Experimental Medicine and Biology·M S KellermayerH L Granzier
Jan 12, 2001·Journal of Molecular Biology·A D LiversageJ Trinick
Feb 13, 2001·Biophysical Journal·M S KellermayerH L Granzier
Jul 17, 2001·Journal of Molecular Biology·L Tskhovrebova, J Trinick
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·H LiJ M Fernandez
Jan 19, 2002·The Journal of Biological Chemistry·Kaori WatanabeHenk Granzier
Mar 26, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·L Tskhovrebova, J Trinick
Jun 4, 2002·The Journal of Physiology·Henk Granzier, Siegfried Labeit

❮ Previous
Next ❯

Citations

Oct 19, 2004·Basic Research in Cardiology·Friedrich K SchmielHans-Heiner Kramer
Dec 7, 2007·Pflügers Archiv : European journal of physiology·Wolfgang A Linke, Anika Grützner
Jan 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Eleonore von CastelmurOlga Mayans
Apr 24, 2012·The Journal of Biological Chemistry·Sergi Garcia-ManyesJulio M Fernández
May 13, 2006·Annual Review of Biophysics and Biomolecular Structure·Viola Vogel
Jul 14, 2010·Journal of Biomedicine & Biotechnology·Mark S MillerJim O Vigoreaux
Feb 22, 2011·Annual Review of Biophysics·Jen HsinKlaus Schulten
Jul 14, 2010·Journal of Biomedicine & Biotechnology·Larissa Tskhovrebova, John Trinick
Feb 21, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·C Derek MaNicholas L Abbott
May 23, 2006·Journal of Structural Biology·B KissM S Z Kellermayer
Jun 12, 2013·Biophysical Journal·Árpád KarsaiSamantha P Harris
Apr 26, 2005·Biophysical Journal·A NagyM S Z Kellermayer
Dec 13, 2006·Biophysical Journal·Atom SarkarJulio M Fernandez
Feb 9, 2006·Journal of Muscle Research and Cell Motility·Larissa TskhovrebovaJohn Trinick
Dec 20, 2003·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Jack LidmarDavid R Nelson
Aug 28, 2003·Nature Structural Biology·Andreas Matouschek, Carlos Bustamante
Sep 16, 2006·Biopolymers·A PaananenT J McMaster
Jan 23, 2019·Protein Science : a Publication of the Protein Society·Jacob A WhitleyNathan T Wright
May 12, 2005·Physiological Measurement·Miklós S Z Kellermayer
Apr 8, 2020·Frontiers in Physiology·Thomas LanzicherHenk Granzier
Mar 21, 2008·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·S MarchettiA Toscano
Jan 27, 2019·Journal of Biological Engineering·Shanshan YuJiangguo Lin
Dec 15, 2004·The Journal of Biological Chemistry·Miklós S Z KellermayerBotond Penke
May 2, 2018·Scientific Reports·Chunhua DongMichael T Woodside
Mar 28, 2019·Scientific Reports·Sivaraman RajaganapathyMurti V Salapaka
Sep 28, 2010·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Hyungsuk LeeRoger D Kamm
Dec 21, 2013·Journal of Cell Science·Zsolt MártonfalviMiklós Kellermayer
Sep 4, 2021·Biomechanics and Modeling in Mechanobiology·Christopher J MorrisJames E Moore

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.