PMID: 2492499Jan 1, 1989Paper

Mechanism and energetics of dipeptide transport in membrane vesicles of Lactococcus lactis

Journal of Bacteriology
E J SmidW N Konings

Abstract

Alanyl-alpha-glutamate transport has been studied in Lactococcus lactis ML3 cells and in membrane vesicles fused with liposomes containing beefheart cytochrome c oxidase as a proton-motive-force-generating system. The uptake of Ala-Glu observed in de-energized cells can be stimulated 26-fold upon addition of lactose. No intracellular dipeptide pool could be detected in intact cells. In fused membranes, a 40-fold accumulation of Ala-Glu was observed in response to a proton motive force. Addition of ionophores and uncouplers resulted in a rapid efflux of the accumulated dipeptide, indicating that Ala-Glu accumulation is directly coupled to the proton motive force as a driving force. Ala-Glu uptake is an electrogenic process and the dipeptide is transported in symport with two protons. In both fused membranes and intact cells the same affinity constant (0.70 mM) for Ala-Glu uptake was found. Accumulated Ala-Glu is exchangeable with externally added alanyl-glutamate, glutamyl-glutamate, and leucyl-leucine, while no exchange occurred upon addition of the amino acid glutamate or alanine. These results indicate that the Ala-Glu transport system has a broad substrate specificity.

References

Mar 1, 1978·Journal of General Microbiology·B A Law
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A J DriessenW Konings
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A J DriessenW N Konings
Feb 3, 1986·European Journal of Biochemistry·A J DriessenW N Konings
Mar 1, 1988·Infection and Immunity·A H RogersA L Pfennig
Jan 1, 1986·Methods in Enzymology·C F Higgins, M M Gibson
Oct 1, 1974·Journal of Bacteriology·J L Cowell
Jul 15, 1981·Analytical Biochemistry·Y TapuhiB L Karger
Jan 1, 1986·Applied and Environmental Microbiology·A van Boven, W N Konings
Dec 1, 1987·Applied and Environmental Microbiology·A van Boven, W N Konings

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Citations

May 1, 1993·The Journal of Dairy Research·P S TanW N Konings
May 1, 1996·The Journal of Dairy Research·H LaanW N Konings
Aug 1, 2012·Bioprocess and Biosystems Engineering·Ozlem Ustün, Gaye Ongen
Sep 1, 1993·FEMS Microbiology Reviews·B Poolman
Oct 17, 2001·The Journal of Biological Chemistry·Peggy GaraultVeronique Monnet
Jul 31, 2012·The EMBO Journal·Poul Nissen
Sep 1, 1993·FEMS Microbiology Reviews·G G Pritchard, T Coolbear
Nov 1, 1995·Journal of Periodontal Research·J Tang-LarsenJ Carlsson
Mar 4, 2014·Annual Review of Food Science and Technology·E J Smid, M Kleerebezem
Sep 1, 1990·FEMS Microbiology Reviews·J Kok
Oct 1, 1996·Antonie van Leeuwenhoek·E R KunjiW N Konings
Apr 18, 1998·Applied and Environmental Microbiology·V JuillardJ C Gripon
Mar 1, 1989·Journal of Bacteriology·A J DriessenW N Konings
Dec 1, 1989·Applied and Environmental Microbiology·H Laan, W N Konings
Aug 1, 1995·Applied and Environmental Microbiology·V JuillardJ Richard
Sep 1, 1991·Applied and Environmental Microbiology·E J SmidW N Konings
Dec 1, 1991·Applied and Environmental Microbiology·P S TanW N Konings
Feb 1, 1990·Applied and Environmental Microbiology·P S Tan, W N Konings

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