Jan 1, 1989

Mechanism of action of 5-AZA-dC: induced DNA hypomethylation does not lead to aberrant gene expression in human leukemic CEM cells

Leukemia Research
J Bouchard


To understand the mechanism of action of 5-AZA-2'-deoxycytidine, a potent antineoplastic agent, we studied its effect in CEM cells on DNA methylation while monitoring coinciding changes in DNA, RNA and protein synthesis. At concentrations near the inhibitory concentration, the drug induced a profound reduction of DNA methylation. Effects on DNA and RNA synthesis were also noted at 24 and 48 h of treatment. However, at all concentrations assayed, no change in polypeptide composition as monitored by 2-D gel electrophoresis, were observed. That the cytotoxic effect of 5-AZA-deoxycytidine is due to changes in gene expression induced by DNA methylation reduction is not supported.

Mentioned in this Paper

Leukemia, Lymphocytic, Acute, L2
Antineoplastic Agents
DNA, Neoplasm
Gene Expression
Hairy Cell Leukemia
RNA, Neoplasm
Genotoxic Stress
Neoplasm Proteins

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