PMID: 6809046Jun 22, 1982

Mechanism of action of cutinase: chemical modification of the catalytic triad characteristic for serine hydrolases

Biochemistry
W Köller, P E Kolattukudy

Abstract

Cutinase from Fusarium solani f. sp. pisi was inhibited by diisopropyl fluorophosphate and phenylboronic acid, indicating the involvement of an active serine residue in enzyme catalysis. Quantitation of the number of phosphorylated serines showed that modification of one residue resulted in complete loss of enzyme activity. One essential histidine residue was modified with diethyl pyrocarbonate. This residue was buried in native cutinase and became accessible to chemical modification only after unfolding of the enzyme by sodium dodecyl sulfate. The modification of carboxyl groups with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide in the absence of sodium dodecyl sulfate did not result in inactivation of the enzyme; however, such modifications in the presence of sodium dodecyl sulfate resulted in complete loss of enzyme activity. The number of residues modified was determined by incorporation of [14C]glycine ethyl ester. Modification of cutinase in the absence of sodium dodecyl sulfate and subsequent unfolding of the enzyme with detergent in the presence of radioactive glycine ester showed that one buried carboxyl group per molecule of cutinase resulted in complete inactivation of the enzyme. Three additional peripheral carbox...Continue Reading

References

Aug 12, 1983·Biochemical and Biophysical Research Communications·C Soliday, P E Kolattukudy
Jan 1, 1987·The International Journal of Biochemistry·R J Foster, P E Kolattukudy
Jun 11, 1992·Enzyme and Microbial Technology·F X MalcataC H Amundson
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C SolidayP E Kolattukudy
Jan 1, 1992·The Journal of Orthopaedic and Sports Physical Therapy·M E FranklinK Forgione
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·A X Li, J C Steffens
May 20, 2015·Applied Microbiology and Biotechnology·Antti Nyyssölä
Mar 1, 1992·Molecular & General Genetics : MGG·J A SweigardB Valent

Citations

Aug 1, 1978·Angewandte Chemie·F Schneider
Nov 15, 1979·Journal of Molecular Biology·A R SieleckiM N James
Jan 1, 1977·Annual Review of Biochemistry·J Kraut
Apr 1, 1973·The Biochemical Journal·J J Holbrook, V A Ingram
Apr 1, 1967·Chemical Reviews·F Kurzer, K Douraghi-Zadeh
Nov 1, 1969·Journal of Colloid and Interface Science·M C Carey, D M Small
May 28, 1969·Journal of Molecular Biology·K L CarrawayD E Koshland
Aug 1, 1980·Archives of Biochemistry and Biophysics·D Farb, W P Jencks
May 30, 1980·Science·P E Kolattukudy
Jan 1, 1972·Methods in Enzymology·K L Carraway, D E Koshland

Related Concepts

Benzeneboronic acid
Transverse Tubule of Muscle Cell
Histidine
Cutinase
Isoflurophate
Triad Acrylic Resin
CDIPT
Endopeptidases
Glycine
Plasma Protein Binding Capacity

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.