PMID: 12369908Oct 10, 2002Paper

Mechanism of action of D-xylose isomerase

Current Protein & Peptide Science
B Asbóth, G Náray-Szabó

Abstract

The present knowledge on the stereochemical mechanism of action of glucose (or xylose) isomerase, one of the highest tonnage industrial enzymes, is summarized. First we deal shortly with experimental methods applied to study the structure and function of this enzyme: enzyme kinetics, protein engineering, X-ray crystallography, nuclear magnetic and electron paramagnetic resonance spectroscopy. Computational methods like homology modeling, molecular orbital, molecular dynamics and continuum electrostatic methods are also shortly treated. We discuss mostly those results and their contribution to the elucidation of the mechanism of action that have been published in the last decade. Structural characteristics of free xylose isomerase as well as its complexes with various ligands are depicted. This information provides a tool for the study of structural details of the enzyme mechanism. We present a general mechanism where the first step is ring opening, which is followed by the extension of the substrate to an open-chain conformation, a proton shuttle with the participation of a structural water molecule and the rate-determining hydride shift. The role of metal ions in the catalytic process is discussed in detail. Finally we present...Continue Reading

Citations

May 16, 2012·Protein Engineering, Design & Selection : PEDS·Darshan H PatelHyeun-Jong Bae
Sep 6, 2012·Acta Crystallographica. Section D, Biological Crystallography·Andrey KovalevskyPaul Langan
May 26, 2006·European Biophysics Journal : EBJ·Edward H SnellFlora Meilleur
May 5, 2006·European Biophysics Journal : EBJ·Flora MeilleurDean A A Myles
Jun 4, 2005·Biotechnology Progress·Phillip R GibbsAndreas S Bommarius
May 19, 2009·Journal of Chromatography. a·Zhongqiao Hu, Jianwen Jiang
Apr 26, 2008·Journal of Molecular Biology·Cong LiuXiao-Dong Su
Oct 19, 2010·Protein Science : a Publication of the Protein Society·Louise J GourlayMartino Bolognesi
Apr 8, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Esko OksanenSuzanne Zoë Fisher
Feb 18, 2014·Acta Crystallographica. Section D, Biological Crystallography·Parthapratim MunshiFlora Meilleur
Sep 5, 2017·Nature Chemical Biology·Thomas ThomikMislav Oreb
Jul 6, 2019·Journal of Synchrotron Radiation·Elspeth F Garman, Martin Weik
Jul 6, 2019·Journal of Synchrotron Radiation·Helena TabermanElspeth F Garman
Feb 26, 2013·Chemical Society Reviews·Robert DiCosimoGregory Bohlmann
Feb 11, 2009·Acta Biochimica Et Biophysica Sinica·Ming DingFukun Zhao
Jan 1, 2013·Biomolecules·Gábor Náray-SzabóBalázs Krámos
Feb 9, 2020·Biochimica Et Biophysica Acta. General Subjects·Renan Yuji MiyamotoLeticia Maria Zanphorlin
Aug 10, 2006·Chemical Reviews·Jiali GaoDonald G Truhlar

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