PMID: 6487790Sep 1, 1984

Mechanism of action of the stem-cell inhibition factor on the formation of exogenous hemopoietic colonies in the spleen of mice

Biulleten' eksperimental'noĭ biologii i meditsiny
O Iu Ignat'evaT A Golovanova

Abstract

It was established by previous works that thymocytes treated with antilymphocyte serum secrete soluble factor capable of inhibiting exogenous colony formation in the spleen of lethally irradiated mice injected with bone marrow cells treated with the stem cell inhibition factor (SCIF). The purpose of the present investigation was to explore possible mechanisms of SCIF action. Regeneration of erythropoiesis (measured by 59Fe incorporation) in the spleen and bone marrow of mice injected with SCIF-treated bone marrow cells was inhibited as compared with control, while CFUs started proliferating with a 3-day delay. Two hours after SCIF treatment 60% of CFUs entered S phase as judged by hydroxyurea cell kill. The CFUs fraction treated with the SCIF was found to be diminished 3-4-fold as compared with control. The data obtained suggest that SCIF treatment makes CFUs enter 3 phase, which may account for the reduced capacity of CFUs to populate the spleen and to proliferate with a 3-day delay.

Related Concepts

Stem cell inhibitory factor
Marrow
Bone Marrow Cells
Colony-Forming Units Assay
Hematopoiesis, Medullary
Colony-Forming Units, Hematopoietic
Lymphokines
Mice, Inbred CBA
Mice, Inbred C57BL
Spleen

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