PMID: 8948504Nov 1, 1996Paper

Mechanism of action of TNF-alpha-stimulated prostaglandin production in cultured bovine luteal cells

Prostaglandins
David H Townson, J L Pate

Abstract

Tumor necrosis factor-alpha (TNF-alpha) influences hormone synthesis of many ovarian cell types and can also exert cytotoxic effects, possibly by increasing the synthesis of prostaglandins. The purpose of the present study was to characterize the mechanism of TNF-alpha-stimulated prostaglandin; F2 alpha (PGF2 alpha) production in cultured bovine luteal cells. Inhibitors of RNA and protein synthesis (actinomycin D and cycloheximide, respectively) completely blocked TNF-alpha-stimulated PGF2 alpha production. The phospholipase A2 inhibitor, aristolochic acid, prevented TNF-alpha-stimulated, but not basal, PGF2 alpha production, whereas the phospholipase C inhibitor, compound 48/80, was without effect. The addition of arachidonic acid to cultures did not overcome the inhibitory effects of cycloheximide or aristolochic acid. In conclusion, TNF-alpha-stimulated prostaglandin production by bovine luteal cells is dependent upon the stimulation of phospholipase A2 through mechanisms which require synthesis of RNA and protein.

References

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Citations

Mar 1, 2000·Molecular Reproduction and Development·R SakumotoK Okuda
Jun 5, 2002·Molecular and Cellular Endocrinology·F J DiazM C Wiltbank
May 29, 2008·Proceedings of the National Academy of Sciences of the United States of America·Luiz E HenkesBo R Rueda
Nov 14, 2003·Reproductive Biology and Endocrinology : RB&E·Milo C Wiltbank, Joseph S Ottobre
Nov 14, 2003·Reproductive Biology and Endocrinology : RB&E·Kiyoshi Okuda, Ryosuke Sakumoto
Feb 3, 2007·Reproductive Biology and Endocrinology : RB&E·Matthew J CannonJoy L Pate
Mar 10, 2004·The Journal of Reproduction and Development·Ryosuke Sakumoto, Kiyoshi Okuda
Sep 11, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Daniel H PooleJoy L Pate
Jul 10, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Matthew J CannonJoy L Pate
Oct 21, 2015·Prostaglandins & Other Lipid Mediators·Ilona Kowalczyk-ZiebaIzabela Woclawek-Potocka
Mar 26, 2013·Reproduction in Domestic Animals = Zuchthygiene·L M ZorrillaJ E Gadsby
Sep 23, 2016·Domestic Animal Endocrinology·J ChangJ E Gadsby
Oct 7, 2011·Biology of Reproduction·Daniel H Poole, Joy L Pate
Feb 9, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·S S WalusimbiJ L Pate
Feb 21, 2013·Journal of Animal Science·S S Walusimbi, J L Pate

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