Mechanism of activation of Xenopus CFTR by stimulation of PKC

American Journal of Physiology. Cell Physiology
Yongyue ChenL Reuss

Abstract

PKA-mediated phosphorylation of the regulatory (R) domain plays a major role in the activation of the human cystic fibrosis transmembrane conductance regulator (hCFTR). In contrast, the effect of PKC-mediated phosphorylation is controversial, smaller than that of PKA, and dependent on the cell type. In the present study, we expressed Xenopus CFTR (XCFTR) and hCFTR in Xenopus oocytes and examined their responses (i.e., macroscopic membrane conductance) to maximal stimulation by PKC and PKA agonists. With XCFTR, the average response to PKC was approximately sixfold that of PKA stimulation. In contrast, with hCFTR, the response to PKC was approximately 90% of the response to PKA stimulation. The reason for these differences was the small response of XCFTR to PKA stimulation. Using the substituted cysteine accessibility method, we found no evidence for insertion of functional CFTR channels in the plasma membrane in response to PKC stimulation. The increase in macroscopic conductance in response to PKC stimulation of XCFTR was due to an approximately fivefold increase in single-channel open probability, with a minor (approximately 30%) increase in single-channel conductance. The responses of XCFTR to PKC stimulation and of hCFTR to ...Continue Reading

References

Sep 1, 1994·The Journal of General Physiology·H Fischer, T E Machen
Jan 1, 1995·American Journal of Respiratory and Critical Care Medicine·D A SchwartzJ A Merchant
Dec 1, 1996·The American Journal of Physiology·A TakahashiR A Frizzell
Jun 24, 1998·Pflügers Archiv : European journal of physiology·H FischerT E Machen
Aug 5, 1998·The American Journal of Physiology·L M Middleton, R D Harvey
Jan 29, 1999·Physiological Reviews·D N Sheppard, M J Welsh
Jan 29, 1999·Physiological Reviews·D C DawsonM K Mansoura
Apr 28, 1999·Advances in Second Messenger and Phosphoprotein Research·D C Gadsby, A C Nairn
Jul 10, 1999·The Journal of Membrane Biology·W M Weber
Oct 16, 1999·Pflügers Archiv : European journal of physiology·W M WeberW Van Driessche
Aug 2, 2001·The Journal of General Physiology·P ChenK D Gillis
Jun 5, 2003·American Journal of Physiology. Cell Physiology·Carol A Bertrand, Raymond A Frizzell
Jul 30, 2004·American Journal of Physiology. Cell Physiology·Yongyue ChenLuis Reuss

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Citations

Mar 16, 2005·The Journal of General Physiology·Silvia G BompadreTzyh-Chang Hwang
Apr 22, 2008·Journal of Molecular Biology·Tamás HegedusJohn R Riordan
Jul 30, 2004·American Journal of Physiology. Cell Physiology·Yongyue ChenLuis Reuss
Oct 26, 2005·Journal of Cellular Physiology·Melissa L PalmerScott M O'Grady
Sep 15, 2005·American Journal of Physiology. Renal Physiology·Clint PerryIrina I Grichtchenko

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