PMID: 8582138Aug 1, 1995Paper

Mechanism of amikacin resistance in Pseudomonas aeruginosa isolates from patients with cystic fibrosis

Diagnostic Microbiology and Infectious Disease
J C HurleyA L Smith

Abstract

We studied 27 amikacin-resistant isolates of Pseudomonas aeruginosa from patients with cystic fibrosis to determine the mechanism of antibiotic resistance. The absence of aminoglycoside-modifying enzymes (AMEs) in these isolates was inferred from the failure of DNA probes for 16 candidate AMEs to hybridize with DNA harvested from these isolates and, in addition, the uniform reduction in susceptibility to a panel of aminoglycosides. In eight of the 27 isolates that were resistant to amikacin at high levels (minimum inhibitory concentration > or = 250 micrograms/ml), plasmids were not detected. The ribosomes of these isolates were sensitive to amikacin in studies of protein synthesis by cell "ghosts." These data suggest that impermeability is the mechanism of amikacin resistance in isolates of P. aeruginosa from patients with cystic fibrosis. Recognition of this mode of resistance may be difficult, as some isolates appeared to be borderline susceptible when tested against aminoglycosides other than amikacin, or had zone diameters that overlapped those obtained with amikacin-susceptible isolates.

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Citations

Dec 30, 2006·Antimicrobial Agents and Chemotherapy·Farid El'GarchPatrick Plésiat
Mar 5, 2009·Antimicrobial Agents and Chemotherapy·Lucie VettorettiCatherine Llanes
Jun 28, 2006·Antimicrobial Agents and Chemotherapy·Julie M Struble, Ryan T Gill
Jan 28, 2005·Antimicrobial Agents and Chemotherapy·Keith Poole
Jun 17, 2009·Journal of Medical Microbiology·Tanya Strateva, Daniel Yordanov
Aug 14, 2009·FEMS Microbiology Letters·Anthony M GeorgePeter G Middleton
Sep 18, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S IslamB Wretlind
Jan 22, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S IslamB Wretlind
May 26, 2005·The Journal of Antimicrobial Chemotherapy·Keith Poole
Jan 23, 2019·Frontiers in Cellular and Infection Microbiology·July FongMichael Givskov

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