Mechanism of an amphipathic alpha-helical peptide's antiviral activity involves size-dependent virus particle lysis.

ACS Chemical Biology
Nam-Joon ChoJeffrey S Glenn

Abstract

The N-terminal region of the hepatitis C virus (HCV) nonstructural protein NS5A contains an amphipathic alpha-helix that is necessary and sufficient for NS5A membrane association. A synthetic peptide (AH) comprising this amphipathic helix is able to lyse lipid vesicles that serve as a model system for virus particles. Based on quartz crystal microbalance-dissipation (QCM-D) experiments, the degree of vesicle rupturing was found to be inversely related to vesicle size, with maximal activity in the size range of several medically important viruses. In order to confirm and further study vesicle rupture, dynamic light scattering (DLS) and atomic force microscopy (AFM) experiments were also performed. The size dependence of vesicle rupturing helps explain the peptide's observed effect on the infectivity of a wide range of viruses. Further, in vitro studies demonstrated that AH peptide treatment significantly decreased the infectivity of HCV particles. Thus, the AH peptide might be used to rupture HCV particles extra-corporally (for HCV prevention) and within infected individuals (for HCV therapy).

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Citations

May 17, 2012·Biointerphases·Joshua A Jackman, Nam-Joon Cho
Oct 18, 2014·Cellular and Molecular Life Sciences : CMLS·Jill M Steinbach
Jan 12, 2016·Journal of the American Chemical Society·Joshua A JackmanNam-Joon Cho
Jan 8, 2016·Physical Chemistry Chemical Physics : PCCP·Min Chul KimNam-Joon Cho
Nov 26, 2010·Trends in Molecular Medicine·Michael A Gelman, Jeffrey S Glenn
Aug 4, 2011·Journal of Molecular Recognition : JMR·Bernd Becker, Matthew A Cooper
Aug 25, 2015·Biomaterials·Jinjin ZhangNatalia A Osna
Sep 14, 2013·Current Opinion in Colloid & Interface Science·Gregory J HardyStefan Zauscher
May 16, 2015·Chemical Communications : Chem Comm·Seyed R Tabaei, N J Cho
Dec 18, 2014·European Biophysics Journal : EBJ·Maria WallinMartin Andersson
May 14, 2016·Physical Chemistry Chemical Physics : PCCP·Seong-Oh KimNam-Joon Cho
Aug 28, 2014·Analytical Chemistry·Jinghui WangSibani Lisa Biswal
Apr 19, 2018·The Analyst·Meriem ChadliAgnès Girard-Egrot
Jul 1, 2018·Chemistry, an Asian Journal·Joshua A JackmanKatsuhiko Ariga
Jun 6, 2019·Nanomedicine·Noura H Abd EllahHelal F Hetta
Sep 4, 2020·ChemPlusChem·Lili LiangGrzegorz Lisak
Oct 26, 2018·Nature Materials·Jing Zou, Pei-Yong Shi
Oct 24, 2018·Nature Materials·Joshua A JackmanNam-Joon Cho
Dec 24, 2019·Frontiers in Microbiology·Lili WangFei Yu
Sep 11, 2020·Journal of Virology·Andrew R HoffmannWilliam C Wimley
Feb 2, 2021·Nano Today·Jiarong ZhouLiangfang Zhang
May 18, 2013·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Vladimir P Zhdanov, Fredrik Höök
Jul 30, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·J BompardA Girard-Egrot
Nov 28, 2013·The Journal of Physical Chemistry. B·Joshua A JackmanNam-Joon Cho
Jul 11, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Soohyun ParkNam-Joon Cho
Mar 19, 2014·The Journal of Physical Chemistry. B·Goh Haw ZanNam-Joon Cho
Feb 27, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Angélique CoutableEmmanuelle Trévisiol
Sep 7, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Meriem ChadliAgnès Girard-Egrot
Jan 10, 2019·Chemical Reviews·Shantanu GuhaWilliam C Wimley
Mar 12, 2019·ACS Applied Materials & Interfaces·Soohyun ParkNam-Joon Cho
Nov 2, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Choon-Peng ChngChangjin Huang
Jan 29, 2022·Archives of Virology·Rohan Janardhan MeshramRajesh Nivarti Gacche

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