Mechanism of Anti-Cancer Activity of Benomyl Loaded Nanoparticles in Multidrug Resistant Cancer Cells

Journal of Biomedical Nanotechnology
Sudarshan KiniDulal Panda

Abstract

Polymeric chitosan-poly(D,L-lactide-co-glycolide) nanoparticles loaded with benomyl as anticancer drug formulation against multidrug-resistant EMT6/AR1 cells were synthesized by amine-carboxylate reaction. Using transmission electron microscopy, the average size of chitosan-poly(D,L-lactide-co-glycolide) nanoparticles and benomyl-encapsulated polymeric chitosan-poly(D,L-lactide-co-glycolide) nanoparticles was estimated to be 155 ± 20 nm and 160 ± 25 nm, respectively. Fourier transform infrared spectroscopy revealed that poly(D,L-lactide-co-glycolide) and chitosan are linked by covalent bonds. Zeta potentials of benomyl-encapsulated polymeric chitosan-poly(D,L-lactide-co-glycolide) nanoparticles at pH 4, 7.2, and 10 were 30 ± 1.8, 19 ± 0.65, and -22 ± 0.15 mV, respectively, indicating the formation of stable, hydrophilic nanoparticles. The release of benomyl from benomyl-encapsulated polymeric chitosan-poly(D,L-lactide-co-glycolide) nanoparticles followed pH-dependent kinetics. The uptake of fluorescein isothiocyanate-labeled chitosan-poly(D,L-lactide-co-glycolide) nanoparticles was concentration-dependent in both MCF-7 and multidrug-resistant EMT6/AR1 cells. EMT6/AR1 cells showed 10-fold higher resistance to benomyl compared to...Continue Reading

Citations

Jan 15, 2016·International Journal of Pharmaceutics·Bo ZhuangHuashi Guan
Nov 26, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sérgio R S VelosoElisabete M S Castanheira
May 28, 2020·International Journal of Molecular Sciences·Beatriz D CardosoPaulo J G Coutinho
Mar 2, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Sérgio R S VelosoPaula M T Ferreira
May 1, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Kara MehtapOzhan Gul

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