PMID: 8605586Apr 15, 1996Paper

Mechanism of anti-HIV activity of negatively charged albumins: biomolecular interaction with the HIV-1 envelope protein gp120

Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association
M E KuipersD K Meijer

Abstract

A novel class of polyanionic proteins with potent anti-human immunodeficiency virus type 1 activity, the negatively charged albumins (NCAs), have been reported previously. In vitro antiviral assays established that these compounds preferentially inhibit virus-cell fusion and syncytium formation and that virus-cell binding is less affected. Here the interaction of the NCAs with synthetic peptides composed of 15-36 amino acids and corresponding to different parts of the gp120 envelope protein is described. Among the gp120 peptides tested, binding of the NCAs was observed only with the s0-called V3 loop (amino acids 296-330) and the C-terminal part of gp120. A higher number of negatively charged residues in the albumins resulted in higher binding affinities. NCAs in which, in addition to negative charges, up to 7 or 14 lactose or mannose groups were introduced, respectively did not exhibit increasing binding affinity. In contrast, mannosylated albumin containing about 14 mannose groups showed an increased binding compared with native albumin. Binding of the NCAs to the V3 and C-terminal oligopeptide was competitively inhibited by sulfated polysaccharide heparin and dextran sulfate. This finding indicates that the binding between t...Continue Reading

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Citations

Jan 1, 1996·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·E De Clercq
Apr 4, 1997·Biochemical Pharmacology·P SchoenP J Swart
Mar 23, 1999·Biochemical Pharmacology·M E KuipersH Huisman
May 13, 1999·Antiviral Research·M E BursteinA G Bukrinskaya
Jun 28, 2012·Biochemical and Biophysical Research Communications·Xiaoguang LiEiichi N Kodama
Oct 13, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Johannes H ProostDirk K F Meijer
Sep 7, 2005·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Masaki TeradaRobert Mastico
Apr 4, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Chu WangXiang-Hui Yu
Aug 19, 2000·Antiviral Chemistry & Chemotherapy·M Lüscher-Mattli
Jan 11, 2000·Journal of Peptide Science : an Official Publication of the European Peptide Society·P J SwartD K Meijer
Apr 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L BeljaarsK Poelstra
May 20, 1997·AIDS Research and Human Retroviruses·P J SwartD K Meijer
Mar 23, 2017·Assay and Drug Development Technologies·Charline GiroudGregory B Melikyan

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