Mechanism of antibacterial activity of liposomal linolenic acid against Helicobacter pylori

PloS One
Sung Woo JungMarygorret Obonyo

Abstract

Helicobacter pylori infects approximately half of the world population and is a major cause of gastritis, peptic ulcer, and gastric cancer. Moreover, this bacterium has quickly developed resistance to all major antibiotics. Recently, we developed a novel liposomal linolenic acid (LipoLLA) formulation, which showed potent bactericidal activity against several clinical isolated antibiotic-resistant strains of H. pylori including both the spiral and coccoid form. In addition, LipoLLA had superior in vivo efficacy compared to the standard triple therapy. Our data showed that LipoLLA associated with H. pylori cell membrane. Therefore, in this study, we investigated the possible antibacterial mechanism of LipoLLA against H. pylori. The antibacterial activity of LipoLLA (C18:3) was compared to that of liposomal stearic acid (LipoSA, C18:0) and oleic acid (LipoOA, C18:1). LipoLLA showed the most potent bactericidal effect and completely killed H. pylori within 5 min. The permeability of the outer membrane of H. pylori increased when treated with LipoOA and LipoLLA. Moreover, by detecting released adenosine triphosphate (ATP) from bacteria, we found that bacterial plasma membrane of H. pylori treated with LipoLLA exhibited significantly...Continue Reading

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Citations

Jan 16, 2016·The Korean Journal of Internal Medicine·Sung Woo Jung, Sang Woo Lee
Mar 8, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Joshua A JackmanNam-Joon Cho
Sep 2, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Bo Kyeong YoonNam-Joon Cho
Apr 20, 2017·Critical Reviews in Microbiology·Bushra Jamil, Muhammad Imran
Jan 16, 2017·International Journal of Pharmaceutics·Catarina Leal SeabraM Cristina L Martins
Feb 8, 2020·Nanomedicine·Qianyu ZhangXuefeng Xia
Oct 16, 2019·Journal of Drug Targeting·Qianyu ZhangXuefeng Xia
Jun 4, 2020·Antibiotics·Enzo IerardiAlfredo Di Leo
Apr 13, 2018·International Journal of Molecular Sciences·Bo Kyeong YoonNam-Joon Cho
Apr 21, 2020·Applied Microbiology and Biotechnology·Qingqing ZhouQing Gu
Feb 25, 2021·International Journal of Biological Macromolecules·Melappa GovindappaArivalagan Pugazhendhi
Mar 17, 2021·Critical Reviews in Microbiology·Maurício Palmeira Chaves de SouzaMarlus Chorilli
Jun 13, 2016·ACS Biomaterials Science & Engineering·Hong-Yin WangFu-Gen Wu
Oct 14, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ioana Alexandra CardosSimona Cavalu

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Methods Mentioned

BETA
dynamic light scattering

Software Mentioned

GraphPad Prism
GraphPad

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