Mechanism of antibody-mediated reduction of nasopharyngeal colonization by Haemophilus influenzae type b studied in an infant rat model

The Journal of Infectious Diseases
M Kauppi-KorkeilaH Käyhty

Abstract

The mechanism of antibody-mediated reduction of Haemophilus influenzae type b (Hib) carriage was studied in the infant rat colonization model. Monoclonal Hib polysaccharide (PS) antibody (MAb) given intranasally or intraperitoneally and human secretory anti-Hib PS IgA given intranasally inhibited colonization by Hib during the entire follow-up period (2-48 h after challenge) but did not affect colonization by Hi, a noncapsulated variant of Hib. F(ab')2 fragments, prepared from the MAb or from human serum anti-Hib IgG reduced Hib colonization as efficiently as the uncleaved molecules. Complement depletion by cobra venom treatment had no effect on the antibody-mediated reduction of Hib colonization. These results indicate that Fc-mediated activities of immunoglobulins are not essential in the reduction of Hib colonization. Instead, antibodies to Hib most likely reduce colonization by a direct effect on growth of the bacteria or their adherence to the nasopharyngeal mucosa.

Citations

May 15, 2002·The Pediatric Infectious Disease Journal·Qibo ZhangAdam Finn
Sep 29, 2004·Journal of Clinical Pathology·Q Zhang, A Finn
Aug 21, 2013·Human Vaccines & Immunotherapeutics·Michael E Pichichero
Aug 30, 2013·Clinical and Vaccine Immunology : CVI·Stefan FernandezRobert G Ulrich
Nov 7, 2019·The Journal of Clinical Investigation·Ulrike BinskerJeffrey N Weiser

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