PMID: 6402027Jan 7, 1983Paper

Mechanism of arachidonic acid release in human polymorphonuclear leukocytes

Biochimica Et Biophysica Acta
C E WalshM Waite

Abstract

Previous studies have demonstrated that [3H]arachidonic acid is released from prelabeled human neutrophil phospholipids when the cells are stimulated by calcium ionophore A23187 or by opsonized zymosan. Neither lysophospholipid generated by phospholipase A2 activity, diacylglycerol nor monoacylglycerol produced via phospholipase C/diacylglycerol lipase action have been identified following neutrophil challenge. The inability to detect any intermediates during the release of arachidonate is due to either rapid reacylation of lysophospholipid or conversion of diacylglycerol (monoacylglycerol) to cellular acylglycerols. The addition of exogenous [14C]fatty acid at the time of challenge was employed to determine the involvement of either phospholipase A2 or phospholipase C activities. Neutrophil stimulation with calcium ionophore A23187 resulted in an incorporation of exogenous [14C]arachidonate into phosphatidylinositol and phosphatidylcholine, those phospholipids which specifically release arachidonate. When the saturated fatty acid, [14C]stearate, replaced [14C]arachidonate, very little [14C]fatty acid was incorporated into any of the phospholipid species. Lipid phosphorus measurements revealed no significant mass change in any ...Continue Reading

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Citations

Feb 1, 1989·Klinische Wochenschrift·C WaydhasL Schweiberer
Jan 1, 1987·International Journal of Immunopharmacology·W EnglbergerU Hadding
May 1, 1991·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K Bry, M Hallman
Aug 4, 1999·Critical Reviews in Clinical Laboratory Sciences·E Kaiser
Sep 1, 1988·Cephalalgia : an International Journal of Headache·Y D FragosoO Sjaastad
Jul 1, 1989·Journal of Cellular Biochemistry·V G Mahadevappa, W S Powell
Apr 2, 1984·European Journal of Biochemistry·M BirmelinK Decker
Oct 1, 1989·Circulation Research·J A LopezD D Heistad
Nov 1, 1988·European Journal of Haematology·N Borregaard
Jul 10, 1986·Biochimica Et Biophysica Acta·C A Dise, D B Goodman
Nov 1, 1992·Infection and Immunity·M F ShaioK D Yang
Dec 12, 1991·Biochimica Et Biophysica Acta·M Lindau, B D Gomperts
Apr 1, 1986·Archives of Biochemistry and Biophysics·D M Smith, M Waite
Jun 30, 1987·Biochemical and Biophysical Research Communications·F H Chilton, R C Murphy
Aug 30, 1984·Biochemical and Biophysical Research Communications·J T O'FlahertyR L Wykle

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