PMID: 6166855May 1, 1981Paper

Mechanism of AT base pairs recognition by molecules of dye "Hoechst 33258"

Molekuliarnaia biologiia
M V MikhaĭlovG V Gurskiĭ

Abstract

Experimental studies are reported on the binding of the dye "Hoechst 33 258" to DNA and synthetic polynucleotides. A stereochemical model of binding of the dye to DNA is proposed. According to the model, the molecules of the dye are located in the minor groove of DNA, each covering 4 base pairs. The benzimidazole backbone of the dye tends to form a helix isogeometric to that of DNA in the B form. The specifisity of "Hoechst 33 258" binding to At pairs involves hydrogen bonding of two NH-groups of benzimidazole rings and CH3N+H group of the piperazine ring to O2 oxygenes of thymines and N3 nitrogens of adenines facing the minor groove of the DNA double helix.

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