Mechanism of autolysis of Neisseria gonorrhoeae.

Journal of Bacteriology
B H Hebeler, F E Young

Abstract

The major autolysin(s) of Neisseria gonorrhoeae was solubilized from envelopes by extraction with 2% Triton X-100 containing 0.5 M NaCl. Neither Triton X-100 nor NaCl alone could effectively release the autolysin(s). The major autolysin is N-acetylmuramyl-L-alanine amidase (EC 3.5.1.28). The pH optimum for this reaction was broad, ranging from 5.5 to 8.5. Optimal hydrolysis of peptidoglycan occurred in 2% Triton X-100 in 0.1 M KCl. Attempts to purify the autolysin were unsuccessful. A rapid assay for enzyme activity was developed using radioactive cell walls as a substrate ([3H]diaminopimelic acid).

References

May 1, 1975·Journal of Bacteriology·B H Hebeler, F E Young
Jun 1, 1976·Journal of Bacteriology·B H Hebeler, F E Young
Jul 1, 1974·Applied Microbiology·L J La Scolea, F E Young
May 1, 1971·CRC Critical Reviews in Microbiology·M L Higgins, G D Shockman

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Citations

Jan 1, 1978·CRC Critical Reviews in Microbiology·S A Morse
Jan 1, 1978·Microbiology and Immunology·T MonodaneS Kotani
Jan 5, 2006·Molecular Microbiology·Holly L Hamilton, Joseph P Dillard
Jun 21, 2017·Journal of Bacteriology·Benjamin I BaardaAleksandra E Sikora
Oct 3, 2018·Antimicrobial Agents and Chemotherapy·Jonathan D LenzJoseph P Dillard
Aug 6, 2019·Cell Host & Microbe·Won Jong KimMagdalene So
Dec 1, 1988·Microbiological Reviews·R J DoyleV Vinter
Mar 1, 1978·Antimicrobial Agents and Chemotherapy·E W GoodellA Tomasz
Apr 1, 1981·Antimicrobial Agents and Chemotherapy·L de Repentigny, P L Turgeon
Jun 1, 1980·Infection and Immunity·R S RosenthalR K Sinha
Apr 14, 1978·Biochemical and Biophysical Research Communications·B H HebelerF E Young

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