Mechanism of cellobiose inhibition in cellulose hydrolysis by cellobiohydrolase

Science in China. Series C, Life Sciences
Zhao YueGao Peiji

Abstract

An experimental study of cellobiose inhibition in cellulose hydrolysis by synergism of cellobiohydrolyse I and endoglucanase I is presented. Cellobiose is the structural unit of cellulose molecules and also the main product in enzymatic hydrolysis of cellulose. It has been identified that cellobiose can strongly inhibit hydrolysis reaction of cellulase, whereas it has no effect on the adsorption of cellulase on cellulose surface. The experimental data of FT-IR spectra, fluorescence spectrum and circular dichroism suggested that cellobiose can be combined with tryptophan residue located near the active site of cellobiohydrolase and then form steric hindrance, which prevents cellulose molecule chains from diffusing into active site of cellulase. In addition, the molecular conformation of cellobiohydrolase changes after cellobiose binding, which also causes most of the non-productive adsorption. Under these conditions, microfibrils cannot be separated from cellulose chains, thus further hydrolysis of cellulose can hardly proceed.

Citations

Dec 10, 2014·Current Microbiology·Tamilvendan ManavalanKlaus Heese
Sep 13, 2011·Applied Biochemistry and Biotechnology·Urvi D Kothari, Yoon Y Lee
Jun 5, 2013·Journal of Industrial Microbiology & Biotechnology·Tetsushi KawaiYoshinori Kobayashi
Apr 1, 2014·Preparative Biochemistry & Biotechnology·Akarin BoonsombutiSujitra Wongkasemjit
Oct 19, 2010·Journal of Industrial Microbiology & Biotechnology·Victor T O SantosWalter Carvalho
Jun 10, 2020·International Journal of Molecular Sciences·Mohamad Farhan Mohamad SobriNorhayati Ramli
Feb 3, 2021·Bioprocess and Biosystems Engineering·Rhudith B CabulongWook-Jin Chung

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