PMID: 6303999Apr 1, 1983

Mechanism of chemical manipulation of the heat resistance of Clostridium perfringens spores

The Journal of Applied Bacteriology
Y Ando, T Tsuzuki

Abstract

The mechanism of chemical manipulation of the heat resistance of Clostridium perfringens type A spores was studied. Spores were converted to various ionic forms to base-exchange technique and these spores were heated at 95 degrees C. Of the four ionic forms, i.e. Ca2+, Na+, H+ and native, only hydrogen spores appeared to have been rapidly inactivated at this temperature, when survivors were enumerated on the ordinary plating medium. However, the recovery of the survivors was improved when the plating medium was supplemented with lysozyme, and more dramatically when the heated spores were pretreated with alkali followed by plating in the medium containing lysozyme. In contrast to crucial damage to germination, in particular to spore lytic enzyme, no appreciable amount of DPA was released from the heat-damaged H-spores. These results suggest that a germination system is involved in the thermal inactivation of the ionic forms of spores, and that exchangeable cation load plays a role in protection from thermal damage of the germination system within the spore. An enhancement of thermal stability of spore lytic enzyme in the presence of a high concentration of NaCl was consistent with the hypothesis.

References

Apr 1, 1976·Applied and Environmental Microbiology·G ALDERTONJ K Chen
Feb 1, 1976·Journal of Bacteriology·R S Flowers, D M Adams
Oct 1, 1972·Journal of Bacteriology·R Scherrer, P Gerhardt
Apr 1, 1966·Journal of Bacteriology·L J Rode, J W Foster
Apr 1, 1966·Journal of Bacteriology·L J Rode, J W Foster
Mar 1, 1970·The Journal of Applied Bacteriology·H L Sadoff
Jan 1, 1968·Analytical Biochemistry·Y Rotman, M L Fields
Mar 14, 1969·Science·G ALDERTON, N SNELL
Jan 1, 1980·Microbiology and Immunology·T NishiharaM Kondo
Jan 31, 1963·Biochemical and Biophysical Research Communications·G ALDERTON, N SNELL
Mar 1, 1965·Clinica Chimica Acta; International Journal of Clinical Chemistry·H H NISHI

Citations

Apr 11, 2001·International Journal of Food Microbiology·E Y Wuytack, C W Michiels
Aug 19, 1997·International Journal of Food Microbiology·A PalopS Condón
Jan 1, 1994·Society for Applied Bacteriology Symposium Series·R E MarquisS Y Shin
Oct 9, 2003·Applied and Environmental Microbiology·Noriyuki IguraIsao Hayakawa
Jan 20, 2015·Foodborne Pathogens and Disease·Barbara M Lund, M W Peck
Aug 1, 1994·FEMS Microbiology Reviews·R E Marquis, S Y Shin
Oct 1, 1992·Letters in Applied Microbiology·M W PeckBarbara M Lund

Related Concepts

Calcium
Cations
Clostridium perfringens
Hydrogen
Hydrolase
Sodium
Sodium Chloride, (24)NaCl
Spores, Bacterial
Physicochemical Phenomena

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