PMID: 6986612Jan 11, 1980Paper

Mechanism of codon-anticodon interaction in ribosomes. Direct functional evidence that isolated 30S subunits contain two codon-specific binding sites for transfer RNA

Nucleic Acids Research
S V KirillovY P Semenkov

Abstract

30S subunits were isolated capable to bind simultaneously two molecules of Phe-tRNAPhe (or N-Acetyl-Phe-tRNAPhe), both poly(U) dependent. The site with higher affinity to tRNA was identified as P site. tRNA binding to this site was not inhibited by low concentrations of tetracycline (2 x 10(-5)M) and, on the other hand, N-Acetyl-Phe-tRNAPhe, initially prebound to the 30S.poly(U) complex in the presence of tetracycline, reacted with puromycin quantitatively after addition of 50S subunits. The site with lower affinity to tRNA revealed features of the A site: tetracycline fully inhibited the binding of both Phe-tRNAPhe and N-Acetyl-Phe-tRNAPhe. Binding of two molecules of Phe-tRNAPhe to the 30S.poly(U) complex followed by the addition of 50S subunits resulted in the formation of (Phe)2-tRNAPhe in 75-90% of the reassociated 70S ribosomes. These results prove that isolated 30S subunits contain two physically distinct centers for the binding of specific aminoacyl- (or peptidyl-) tRNA. Addition of 50S subunits results in the formation of whole 70S ribosomes with usual donor and acceptor sites.

References

Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·G W TischendorfG Stöffler
Apr 1, 1972·Archives of Biochemistry and Biophysics·H WeissbachN Brot
May 28, 1971·Journal of Molecular Biology·J P White, C R Cantor
Oct 20, 1966·Biochemical and Biophysical Research Communications·P Leder, H Bursztyn

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Citations

Apr 11, 1988·FEBS Letters·M V RodninaS V Kirillov
Oct 1, 1986·Journal of Biomolecular Structure & Dynamics·S V Kirillov, Semenkov YuP
Jan 11, 1991·Nucleic Acids Research·I V BoniN V Tzareva
Jul 18, 2008·Nucleic Acids Research·Tatyana V BudkevichKnud H Nierhaus
Sep 1, 1981·European Journal of Biochemistry·M SchmittH G Gassen
Nov 20, 1989·European Journal of Biochemistry·M V RodninaS V Kirillov
Aug 15, 1983·European Journal of Biochemistry·H J RheinbergerK H Nierhaus
Jun 15, 1983·Journal of Molecular Biology·S J RoseO C Uhlenbeck
Dec 16, 1980·Biochemical and Biophysical Research Communications·L Y QuinnT R Quinn
Feb 28, 2002·Methods : a Companion to Methods in Enzymology·D JuzumieneP Wollenzien
Nov 1, 1982·European Journal of Biochemistry·R A GrajevskajaE M Saminsky
Feb 28, 2002·The Journal of Biological Chemistry·Connie YarianPaul F Agris
Nov 14, 1997·Science·S JosephH F Noller

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