Mechanism of conjugated linoleic acid and vaccenic acid formation in human faecal suspensions and pure cultures of intestinal bacteria

Microbiology
Freda M McIntoshR J Wallace

Abstract

Faecal bacteria from four human donors and six species of human intestinal bacteria known to metabolize linoleic acid (LA) were incubated with LA in deuterium oxide-enriched medium to investigate the mechanisms of conjugated linoleic acid (CLA) and vaccenic acid (VA) formation. The main CLA products in faecal suspensions, rumenic acid (cis-9,trans-11-CLA; RA) and trans-9,trans-11-CLA, were labelled at C-13, as were other 9,11 geometric isomers. Traces of trans-10,cis-12-CLA formed were labelled to a much lower extent. In pure culture, Bifidobacterium breve NCFB 2258 formed labelled RA and trans-9,trans-11-CLA, while Butyrivibrio fibrisolvens 16.4, Roseburia hominis A2-183T, Roseburia inulinivorans A2-192T and Ruminococcus obeum-like strain A2-162 converted LA to VA, labelled in a manner indicating that VA was formed via C-13-labelled RA. Propionibacterium freudenreichii subsp. shermanii DSM 4902T, a possible probiotic, formed mainly RA with smaller amounts of trans-10,cis-12-CLA and trans-9,trans-11-CLA, labelled the same as in the mixed microbiota. Ricinoleic acid (12-OH-cis-9-18 : 1) did not form CLA in the mixed microbiota, in contrast to CLA formation described for Lactobacillus plantarum. These results were similar to thos...Continue Reading

References

Apr 1, 1995·Letters in Applied Microbiology·C J RumneyC S Stewart
Feb 29, 2000·Archives of Family Medicine·J HauptmanK R Segal
Apr 1, 2000·Applied and Environmental Microbiology·A BarcenillaH J Flint
Dec 7, 2002·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·J Bassaganya-RieraD C Beitz
Dec 21, 2002·Journal of Applied Microbiology·M CoakleyC Stanton
Oct 1, 1964·Journal of Bacteriology·C E POLANS B TOVE
Jan 27, 2004·Journal of Toxicology and Environmental Health. Part a·Sailendra NichenametlaJerry Exon
May 26, 2004·The American Journal of Clinical Nutrition·Michael W Pariza
Nov 4, 2004·Progress in Lipid Research·Klaus W J WahleDino Rotondo
Nov 13, 2004·Antonie van Leeuwenhoek·Joan E EdwardsR John Wallace
Apr 16, 2005·Science·Paul B EckburgDavid A Relman
Jun 16, 2005·JAMA : the Journal of the American Medical Association·Jean-Pierre ChanoineJonathan Hauptman
Nov 29, 2005·Journal of Bioscience and Bioengineering·Jun OgawaSakayu Shimizu
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Alena LiavonchankaMarkus Georg Rudolph
Oct 3, 2006·International Journal of Systematic and Evolutionary Microbiology·Sylvia H DuncanHarry J Flint
Dec 7, 2006·FEMS Microbiology Letters·R John WallaceDelphine Paillard
Apr 3, 2007·Letters in Applied Microbiology·S H DuncanH J Flint

❮ Previous
Next ❯

Citations

Oct 31, 2006·Antonie van Leeuwenhoek·Margarida R G MaiaR John Wallace
Nov 24, 2012·The Proceedings of the Nutrition Society·Wendy R RussellHarry J Flint
Mar 29, 2014·Nutrients·Gianfranca CartaSebastiano Banni
Jan 14, 2011·Annual Review of Medicine·Giovanni MussoMaurizio Cassader
May 31, 2011·International Journal of Food Microbiology·Anne ThierryGwenaël Jan
Jun 3, 2011·ChemSusChem·An PhilippaertsBert F Sels
Sep 10, 2010·Journal of Applied Microbiology·M MacouzetN Robert
Dec 4, 2012·Molecular Nutrition & Food Research·Barbara D PachikianNathalie M Delzenne
Feb 6, 2014·Clinical Anatomy : Official Journal of the American Association of Clinical Anatomists & the British Association of Clinical Anatomists·Yusuf Ozgur CakmakElif Cigdem Kaspar
Jul 30, 2014·Current Opinion in Immunology·Hagit ShapiroEran Elinav
Apr 19, 2016·The British Journal of Nutrition·Anne M HonkanenKevin J Shingfield
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Lara GorissenKatleen Raes
Jul 20, 2016·BioMed Research International·Stefano RaimondiMaddalena Rossi
Aug 18, 2016·Frontiers in Immunology·Abigail BassonFabio Cominelli
Oct 8, 2016·Critical Reviews in Food Science and Nutrition·Sarah J Clements, Simon R Carding
Aug 28, 2016·World Journal of Microbiology & Biotechnology·Clelia Altieri
Jul 27, 2017·Journal of Medicinal Chemistry·Silvia TurroniMarco Candela
Mar 13, 2018·PLoS Neglected Tropical Diseases·Laura-Isobel McCallJair L Siqueira-Neto
May 16, 2017·Microorganisms·Houem RabahGwénaël Jan
Aug 2, 2018·Journal of the Science of Food and Agriculture·Edoardo Capuano, Nicoletta Pellegrini
Feb 19, 2020·Digestive Diseases and Sciences·Wenly RuanJames Versalovic
May 24, 2017·Frontiers in Immunology·Mark N Read, Andrew J Holmes
Aug 31, 2018·Microbiology and Molecular Biology Reviews : MMBR·Ana S SalsinhaLuis M Rodríguez-Alcalá
Apr 1, 2020·Frontiers in Pharmacology·Gabriela Silveira-NunesAna Maria Caetano Faria
Feb 26, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Seung-Ho SeoHong-Seok Son
Sep 8, 2020·Gut Microbes·Julie RodriguezNathalie M Delzenne
Dec 23, 2020·The Proceedings of the Nutrition Society·Sylvia H DuncanWendy R Russell
Apr 23, 2021·Applied Microbiology and Biotechnology·Romain Jeantet, Gwénaël Jan
Oct 7, 2017·Microbiology Spectrum·Melinda A Engevik, James Versalovic

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.