PMID: 8955302Dec 1, 1996Paper

Mechanism of coordinated synthesis of the antagonistic regulatory proteins NifL and NifA of Klebsiella pneumoniae

Journal of Bacteriology
F GovantesE Santero

Abstract

The nifLA operon of Klebsiella pneumoniae codes for the two antagonistic regulatory proteins which control expression of all other nitrogen fixation genes. NifA is a transcriptional activator, and NifL inhibits NifA. The importance of a correct NifL-NifA stoichiometry for efficient regulation of nitrogen fixation genes has been investigated by constructing a strain with an altered nifL-nifA gene dosage ratio, resulting from the integration of an extra copy of nifA. Results showed that a balanced synthesis of both gene products is essential for correct regulation. Effects of mutations provoking translation termination of nifL upstream or downstream of its natural stop codon, combined with overproduction of both proteins when the genes are transcribed and translated from signals of the phi10 gene of the phage T7, showed that, in addition to the previously reported transcriptional polarity, there is translational coupling between nifL and nifA. In spite of the apparently efficient ribosome binding site of nifA, its rate of independent translation is very low. This is due to a secondary structure masking the Shine-Dalgarno sequence of nifA, which could be melted by ribosomes translating nifL. Mutational analysis confirmed the funct...Continue Reading

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Citations

Nov 5, 1999·Annual Review of Microbiology·C E BauerT H Bird
May 23, 2013·Molecular Microbiology·Sofía Muñoz García-MauriñoEduardo Santero
May 22, 2016·Applied and Environmental Microbiology·Ana Isabel PlateroFernando Govantes
Mar 1, 2009·Microbial Biotechnology·Laura Tomás-GallardoBelén Floriano
Apr 22, 2008·Science in China. Series C, Life Sciences·X HuiZ Jiabi
Sep 3, 1999·Nucleic Acids Research·C R BeuzónJ Casadesús
Apr 2, 1999·Applied and Environmental Microbiology·M J HernáezE Santero
Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·T ArcondéguyM Merrick
Jan 16, 2002·Journal of Bacteriology·Paul RudnickChristina Kennedy
Sep 15, 2000·Journal of Bacteriology·M J HernáezE Santero
Aug 4, 2009·Journal of Bacteriology·Ana B HervásEduardo Santero
Dec 11, 1997·Journal of Bacteriology·L HeS Kustu
May 8, 2021·The New Phytologist·José S RufiánJavier Ruiz-Albert

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