PMID: 2123139Jan 1, 1990Paper

Mechanism of crosslinking of proteins by glutaraldehyde. IV: In vitro and in vivo stability of a crosslinked collagen matrix

Connective Tissue Research
D CheungM E Nimni

Abstract

The use of native or reconstituted collagen as a bioprothesis for tissue augmentation requires the introduction of exogenous synthetic crosslinks. The degree of crosslinking determines the rate of resorption or replacement of the implanted materials by the host. Since biophysical and chemical methods to quantify these crosslinks have in general been difficult to evaluate, we have developed in vitro enzymatic approaches which enable us to correlate the degree of crosslinking with the rates of enzymatic degradation. When the number of stable crosslinks formed is large it is essential to partially unfold the collagen fibrils by heating or by exposure to denaturing agents to enhance their susceptibility to hydrolysis. In the present study we demonstrate that increasing the number of reactive amino groups on collagen by coupling 1,6-diaminohexane to carboxyl groups using a water soluble carbodiimide can significantly enhance the number of crosslinks introduced by glutaraldehyde. We also show that the enzymatic method developed correlates well with the biodegradation of radiolabeled crosslinked collagenous tissues implanted subcutaneously in rats.

References

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Citations

Jun 1, 2011·Chinese Journal of Cancer Research = Chung-kuo Yen Cheng Yen Chiu·Wan-Yin ZhaiYuan-Sen Xu
Aug 15, 2013·Dental Materials : Official Publication of the Academy of Dental Materials·Noriko HiraishiJunji Tagami
Dec 7, 2013·Dental Materials : Official Publication of the Academy of Dental Materials·Ana K Bedran-RussoAriene A Leme
Nov 7, 2009·Journal of Dental Research·G V MacedoA K Bedran-Russo
Feb 26, 2014·Connective Tissue Research·Zhenhua TianGuoying Li
Mar 14, 2003·Journal of Biomedical Materials Research. Part a·Bo HanMarcel E Nimni
Jul 12, 2008·Journal of Tissue Engineering and Regenerative Medicine·Amit D BhranyBuddy D Ratner
Jun 30, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Vincent M FriebeNaren R Vyavahare
Jun 16, 2001·Journal of Biomedical Materials Research·J Lovekamp, N Vyavahare
Dec 13, 2006·Operative Dentistry·Jacques E Nör
Aug 16, 2013·Journal of Investigative and Clinical Dentistry·Lavanya Ajay SharmaGeorge J Dias
Jul 17, 2012·Journal of Prosthodontic Research·Hiroshi EgusaKentaro Akiyama
Jun 4, 2016·Journal of Biomedical Materials Research. Part a·Fahimeh Sadat TabatabaeiKeyvan Moharamzadeh
Feb 23, 1999·Pharmaceutical Development and Technology·R G HuangC M Ofner

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