Mechanism of CuO nano-particles on stimulating production of actinorhodin in Streptomyces coelicolor by transcriptional analysis

Scientific Reports
Xiaomei LiuRutao Liu

Abstract

In this research, antibiotic-producing bacteria, Streptomyces coelicolor (S. coelicolor) M145, was exposed to copper oxide (CuO) particles to investigate the effects of nano-particles (NPs) on antibiotic production. Results showed that a higher yield of antibiotics was obtained with smaller particle sizes of CuO NPs. When exposed to 10 mg/L of 40 nm CuO NPs, the maximum amount of actinorhodin (ACT) obtained was 2.6 mg/L after 144 h, which was 2.0-fold greater than that of control. However, the process was inhibited when the concentration of CuO NPs was increased to higher than 20 mg/L. Transcriptome analysis showed that all the genes involved in the ACT cluster were significantly up-regulated after exposure to 10 mg/L NPs, which could be the direct cause of the increase of ACT production. Additionally, some genes related to the generation of acetyl-coA were up-regulated. In this way, CuO NPs led to an increase of secondary metabolites. The mechanism related to these changes indicated that nano-particle‒induced ROS and Cu2+ played synergetic roles in promoting ACT biosynthesis. This is a first report suggesting that CuO NPs had a significant effect on antibiotic production, which will be helpful in understanding the mechanism of...Continue Reading

References

Mar 6, 2007·Molecular Microbiology·Kapil TahlanJustin R Nodwell
Jul 9, 2008·The Journal of Biological Chemistry·Irina BorodinaJens Nielsen
Feb 3, 2009·Environmental Pollution·Wei JiangBaoshan Xing
Nov 21, 2009·Environmental Science & Technology·Angélique Simon-DeckersMarie Carrière
Sep 28, 2010·Chemical Communications : Chem Comm·Carmela AprilePaolo P Pescarmona
Oct 5, 2011·Chemical Research in Toxicology·Sunandan PakrashiAmitava Mukherjee
Nov 19, 2011·FEMS Microbiology Reviews·Joseph R McCormick, Klas Flärdh
Dec 6, 2011·Chemical Research in Toxicology·Masanori HorieHitoshi Iwahashi
Jan 24, 2012·Chemical Research in Toxicology·Dorota NapierskaPeter H Hoet
Mar 9, 2013·Microbiology and Molecular Biology Reviews : MMBR·Gang LiuHuarong Tan
Oct 9, 2013·Molecular BioSystems·Minal Patkari, Sarika Mehra

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scanning electron microscopy

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