PMID: 4506782Sep 1, 1972

Mechanism of DNA replication by highly purified DNA polymerase of chicken embryo

Proceedings of the National Academy of Sciences of the United States of America
J G StavrianopoulosE Chargaff

Abstract

Highly purified DNA polymerase of chicken embryo was used to investigate the mechanism of DNA replication. The templates used included synthetic homopolymer pairs, as well as DNA of bacteriophage f1 and DNA-RNA hybrids prepared by partial or complete transcription of f1 DNA. The evidence suggests that the DNA synthesized is complementary either to the DNA or the RNA strand of the hybrid, depending on the relative lengths of the two strands, with the longer serving as the template and the shorter as the primer. While f1 DNA itself lacks template properties, the f1 DNA-RNA hybrids are efficient templates; and composition studies show the DNA synthesized to be complementary to the DNA strand of the hybrid. These observations suggest a mechanism of DNA replication in which the initial synthesis of a stable primer by RNA polymerase plays a pivotal role.

References

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Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J G StavrianopoulosE Chargaff
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Citations

Jan 1, 1969·Annual Review of Biochemistry·C C Richardson
Jul 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D Baltimore, D Smoler
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J G StavrianopoulosE Chargaff
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J D Karkas
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J D KarkasE Chargaff
Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D BrutlagA Kornberg
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J G StavrianopoulosE Chargaff
Jul 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R RudnerE Chargaff
Apr 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J D KarkasE Chargaff
Jun 1, 1970·European Journal of Biochemistry·P Hausen, H Stein

Related Concepts

Embryo
Manganese
RNA Denaturation
DNA-Directed RNA Polymerase
Entire Embryo
RNA Polymerase Assembly Pathway
DNA, Viral
Polynucleotides
Alkaline Ribonuclease
Hybrids

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