Mechanism of endotoxin-induced reduction in the number of beta-adrenergic receptors in dog livers: role of phospholipase A

Biochemical Medicine
M S LiuG F Kang

Abstract

The role of phospholipase A on the endotoxin-induced reduction in the number of beta-adrenergic receptors in dog liver plasma membranes was investigated. The results show that digestion of control liver plasma membranes with exogenous phospholipase A2 (0.2 unit/200 micrograms protein) decreased the specific binding of (-)-[3H]dihydroalprenolol by 37.3% (P less than 0.01) and reduced the number of receptor sites by 31.7% (P less than 0.05). These decreases in the specific binding and the number of beta-adrenergic receptors were completely reversible by the addition of phosphatidylcholine (0.2 mM). Endotoxin administration (2 hr postendotoxin) decreased the specific binding by 36% (P less than 0.05) and reduced the number of beta-adrenergic receptors by 33% (P less than 0.05), and these decreases were completely reversible by the addition of 0.2 mM phosphatidylcholine. Digestion of control liver membranes with exogenous phospholipase A2 decreased phosphatidylcholine and phosphatidylethanolamine levels by 50.6 and 51.2%, respectively, but increased lysophosphatidylcholine and lysophosphatidylethanolamine levels by 12- and 8.4-fold, respectively. Endotoxin administration decreased phosphatidylcholine and phosphatidylethanolamine co...Continue Reading

Citations

Apr 21, 1993·Molecular and Cellular Biochemistry·R SalgiaM M Sayeed
Feb 6, 1989·Biochimica Et Biophysica Acta·T S RogersJ A Cook
Feb 1, 1988·Metabolism: Clinical and Experimental·E B Rodríguez de Turco, J A Spitzer
Mar 1, 1988·The Journal of Clinical Investigation·E B Rodríguez de Turco, J A Spitzer
Nov 1, 1988·The Journal of Surgical Research·M S LiuS Ghosh
Dec 1, 1995·Anesthesia and Analgesia·M HeesenG Hempelmann

Related Concepts

Metazoa
Canis familiaris
Endotoxins
Liver
Membrane Lipids
Phospholipase
Phospholipases A
Phospholipids
Beta-adrenergic receptor
Endotoxic Shock

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