Mechanism of Escherichia coli inactivation on palladium-modified nitrogen-doped titanium dioxide.

Biomaterials
Pinggui WuJian Ku Shang

Abstract

The cellular responses of Escherichia coli to visible light photocatalysis were characterized by chemical, optical, electron-beam, and surface-force techniques, to elucidate the mechanisms of photocatalytic inactivation of E. coli on PdO/TiON fiber. The characterization techniques included chemical assays, fluorescence microscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and atomic force microscopy (AFM). Fluorescence microscopy using the Live/Dead BacLight kit indicates that the photocatalytic treatment resulted in severe membrane damage to the E. coli cells. SEM, AFM and TEM revealed drastic defects in the morphology and internal sub-structure of the bacterial cells after the treatments. Combining data from our previous reports on the antimicrobial properties of visible-light-activated PdO/TiON photocatalyst, the present results point to oxidative attack from the exterior to the interior of the bacteria by hydroxyl radicals as the primary mechanism of photocatalytic inactivation.

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Citations

Apr 28, 2011·Applied Microbiology and Biotechnology·Howard A FosterAlex Steele
Oct 12, 2012·Journal of Materials Science. Materials in Medicine·Kai LiXuebin Zheng
Oct 1, 2013·Colloids and Surfaces. B, Biointerfaces·Jinhua LiChuanxian Ding
Mar 29, 2014·Biomedical Microdevices·Krzysztof CendrowskiEwa Mijowska
Feb 14, 2016·Biointerphases·Jaione Romero-MangadoRam P Gandhiraman
Aug 1, 2012·Materials Science & Engineering. C, Materials for Biological Applications·Parveen SultanaPapiya Nandy
Apr 21, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Nhiem Tran, Phong A Tran
Feb 18, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Jingtao ZhangJian Ku Shang
Jan 15, 2014·Materials Science & Engineering. C, Materials for Biological Applications·M PadervandM R Gholami
Aug 1, 2012·Carbohydrate Polymers·Liang ZhangDongliang Li
May 25, 2021·Advanced Materials·Alexey V GulyukAlbena Ivanisevic

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