Mechanism of fibrosis inhibition in laser induced choroidal neovascularization by doxycycline

Experimental Eye Research
Xuening PengXiaoyan Ding

Abstract

To explore the mechanisms underlying doxycycline suppression of fibrosis in laser-induced choroidal neovascularization (LCNV), C57BL/6J male mice (aged from 6 to 8 weeks) received intraperitoneal injections of PBS/doxycycline solution from one day before laser injury until they were sacrificed. Leakage was assessed by FA, and CNV (stained by IB4) or fibrosis (stained by collagen type I) size was measured. The percentage of Pan-keratin+α-SMA+ cells was counted in the eyes' cryostat sections by immunohistochemistry. qPCR was used to measure the mRNA of markers of pan-macrophages, M1 and M2-type macrophages (M1 and M2), markers of EMT, and markers in the downstream of STAT6 signaling. Western blotting was used to analyze the expression of Arg-1, α-SMA, E-cadherin, pSTAT6 and STAT6. Our data showed that doxycycline inhibited leakage from CNV, areas of CNV on day 7 and day 14, and suppressed fibrosis, and the ratio of fibrotic/angiogenic areas during day 7 to day 35. We also showed attenuation of EMT in the doxycycline group. The percentage of Pan-keratin+α-SMA+ cells was lower in the doxycycline group than in the control group. The mRNA and protein levels of mesenchymal markers were downregulated in the doxycycline group, while the...Continue Reading

Citations

Nov 20, 2018·Journal of Leukocyte Biology·Corneliu SimaMichael Glogauer
Apr 26, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Narsis DaftarianAli Hafezi-Moghadam
Jun 20, 2020·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Qiyu BoXiaodong Sun
Nov 7, 2019·Journal of Ophthalmology·Qiong Yi, Wen-Jin Zou

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