PMID: 30835Nov 1, 1978Paper

Mechanism of fluorometric analysis of tetracycline involving metal complexation

Journal of Pharmaceutical Sciences
S T DayJ K Ma


Tetracycline complexation with calcium and organic ligands was studied using fluorescence, circular dichroism, and solvent extraction methods. The results were used to interpret the mechanism of the commonly used fluorometric methods for the analysis of tetracycline in biological fluids. Tetracycline formed ternary calcium complexes with barbital sodium and L-tryptophan in alkaline solutions. The circular dichroism studies indicate that the calcium ion in these complexes is bound to the C-4 dimethylamino and the C-12a hydroxyl groups of tetracycline. These ternary complexes are strongly fluorescent and can be extracted easily into 1-butanol or ethyl acetate. Based on the characteristics of the ternary complexes and of the tetracycline degradation products, it is concluded that only the active form of tetracycline can be complexed and extracted for fluorescence analysis.


Oct 1, 1976·The Analyst·H Poiger, C Schlatter
Dec 1, 1976·Journal of Pharmaceutical Sciences·E C Newman, C W Frank
Jan 1, 1972·Clinica Chimica Acta; International Journal of Clinical Chemistry·D M WilsonA Stillwell
Aug 1, 1972·Antimicrobial Agents and Chemotherapy·L A MitscherT D Sokoloski
Oct 1, 1969·Journal of Pharmaceutical Sciences·J L Colaizzi, P R Klink
Jun 1, 1972·Biochemical Medicine·M Lever
May 1, 1968·Clinica Chimica Acta; International Journal of Clinical Chemistry·A E AltmannF P Maesen
Oct 1, 1968·Tetrahedron Letters·L A MitscherT D Sokoloski
Apr 4, 1969·Analytical Biochemistry·R G KellyK D Hoyt
Mar 1, 1969·Journal of Pharmaceutical Sciences·L J Leeson, J F Weidenheimer
Jun 1, 1963·Analytical Biochemistry·K H IBSENM R URIST
Aug 1, 1963·Journal of Pharmaceutical Sciences·E G REMMERSA P DOERSCHUK

❮ Previous
Next ❯


Aug 24, 1999·Biotechnology and Bioengineering·X MazurM Fussenegger
Apr 25, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·H J Nelis, A P De Leenheer
Jun 1, 1996·Journal of Pharmaceutical and Biomedical Analysis·M Novák-PékliG Pethó
Feb 3, 1998·Journal of Pharmaceutical and Biomedical Analysis·D S Vienneau, C G Kindberg
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marie Louise LokeJette Tjørnelund
May 9, 2014·Journal of Fluorescence·Elmer-Rico E MojicaFrank V Bright
Jan 1, 2008·Toxicology Mechanisms and Methods·Roya KhosrokhavarAzam Bakhtiarian
Jan 1, 1991·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·L M GolubB R Rifkin
Apr 15, 2010·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Richeng XuanKeka C Biswas
Jun 25, 2005·Journal of Chromatography. a·Collin R AndersonWen-Hsin Wu

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.