PMID: 30835Nov 1, 1978Paper

Mechanism of fluorometric analysis of tetracycline involving metal complexation

Journal of Pharmaceutical Sciences
S T DayJ K Ma

Abstract

Tetracycline complexation with calcium and organic ligands was studied using fluorescence, circular dichroism, and solvent extraction methods. The results were used to interpret the mechanism of the commonly used fluorometric methods for the analysis of tetracycline in biological fluids. Tetracycline formed ternary calcium complexes with barbital sodium and L-tryptophan in alkaline solutions. The circular dichroism studies indicate that the calcium ion in these complexes is bound to the C-4 dimethylamino and the C-12a hydroxyl groups of tetracycline. These ternary complexes are strongly fluorescent and can be extracted easily into 1-butanol or ethyl acetate. Based on the characteristics of the ternary complexes and of the tetracycline degradation products, it is concluded that only the active form of tetracycline can be complexed and extracted for fluorescence analysis.

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Citations

Aug 24, 1999·Biotechnology and Bioengineering·X MazurM Fussenegger
Apr 25, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·H J Nelis, A P De Leenheer
Jun 1, 1996·Journal of Pharmaceutical and Biomedical Analysis·M Novák-PékliG Pethó
Feb 3, 1998·Journal of Pharmaceutical and Biomedical Analysis·D S Vienneau, C G Kindberg
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marie Louise LokeJette Tjørnelund
May 9, 2014·Journal of Fluorescence·Elmer-Rico E MojicaFrank V Bright
Jan 1, 2008·Toxicology Mechanisms and Methods·Roya KhosrokhavarAzam Bakhtiarian
Jan 1, 1991·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·L M GolubB R Rifkin
Apr 15, 2010·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Richeng XuanKeka C Biswas
Jun 25, 2005·Journal of Chromatography. a·Collin R AndersonWen-Hsin Wu

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