Mechanism of human somatic reprogramming to iPS cell

Laboratory Investigation; a Journal of Technical Methods and Pathology
Rika TeshigawaraTakashi Tada

Abstract

Somatic reprogramming to induced pluripotent stem cells (iPSC) was realized in the year 2006 in mice, and in 2007 in humans, by transiently forced expression of a combination of exogenous transcription factors. Human and mouse iPSCs are distinctly reprogrammed into a 'primed' and a 'naïve' state, respectively. In the last decade, puzzle pieces of somatic reprogramming have been collected with difficulty. Collectively, dissecting reprogramming events and identification of the hallmark of sequentially activated/silenced genes have revealed mouse somatic reprogramming in fragments, but there is a long way to go toward understanding the molecular mechanisms of human somatic reprogramming, even with developing technologies. Recently, an established human intermediately reprogrammed stem cell (iRSC) line, which has paused reprogramming at the endogenous OCT4-negative/exogenous transgene-positive pre-MET (mesenchymal-to-epithelial-transition) stage can resume reprogramming into endogenous OCT4-positive iPSCs only by change of culture conditions. Genome-editing-mediated visualization of endogenous OCT4 activity with GFP in living iRSCs demonstrates the timing of OCT4 activation and entry to MET in the reprogramming toward iPSCs. Applic...Continue Reading

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Citations

Nov 24, 2019·Laboratory Investigation; a Journal of Technical Methods and Pathology·Hajime IkeharaAkihiro Umezawa
Sep 18, 2020·Experimental Biology and Medicine·Keri Dame, Alexandre Js Ribeiro
Aug 7, 2019·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Rika TeshigawaraTakashi Tada
Apr 23, 2020·Current Psychiatry Reports·Debamitra DasDimitrios Avramopoulos
Sep 2, 2020·International Journal of Molecular Sciences·Cristina OlgasiAntonia Follenzi
Feb 15, 2019·Laboratory Investigation; a Journal of Technical Methods and Pathology·Momoko InoueAkihiro Umezawa
Dec 2, 2020·International Journal of Molecular Sciences·Romana ZahumenskaErika Halasova
Jun 21, 2020·European Journal of Pharmacology·Jolanta HybiakElżbieta Urasińska
May 21, 2021·Reproduction in Domestic Animals = Zuchthygiene·Piyathip SetthawongTheerawat Tharasanit
Jun 18, 2021·Cell Systems·Nika ShakibaDomitilla Del Vecchio
Jun 22, 2021·Frontiers in Bioengineering and Biotechnology·Mira ChingaleKe Huang
Jul 9, 2021·Computer Methods and Programs in Biomedicine·Guanghui YueBaiying Lei
Jul 25, 2021·International Journal of Molecular Sciences·Ying-Chieh WuŠárka Lehtonen

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