Mechanism of imidazolium ionic liquids toxicity in Saccharomyces cerevisiae and rational engineering of a tolerant, xylose-fermenting strain

Microbial Cell Factories
Quinn DickinsonJeff S Piotrowski

Abstract

Imidazolium ionic liquids (IILs) underpin promising technologies that generate fermentable sugars from lignocellulose for future biorefineries. However, residual IILs are toxic to fermentative microbes such as Saccharomyces cerevisiae, making IIL-tolerance a key property for strain engineering. To enable rational engineering, we used chemical genomic profiling to understand the effects of IILs on S. cerevisiae. We found that IILs likely target mitochondria as their chemical genomic profiles closely resembled that of the mitochondrial membrane disrupting agent valinomycin. Further, several deletions of genes encoding mitochondrial proteins exhibited increased sensitivity to IIL. High-throughput chemical proteomics confirmed effects of IILs on mitochondrial protein levels. IILs induced abnormal mitochondrial morphology, as well as altered polarization of mitochondrial membrane potential similar to valinomycin. Deletion of the putative serine/threonine kinase PTK2 thought to activate the plasma-membrane proton efflux pump Pma1p conferred a significant IIL-fitness advantage. Conversely, overexpression of PMA1 conferred sensitivity to IILs, suggesting that hydrogen ion efflux may be coupled to influx of the toxic imidazolium cation....Continue Reading

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Citations

Nov 14, 2016·Applied Microbiology and Biotechnology·Chaowei YuJean S VanderGheynst
Nov 16, 2016·Applied Biochemistry and Biotechnology·Ryosuke YamadaAkihiko Kondo
Apr 11, 2017·ChemSusChem·Susana P F CostaM Lúcia M F S Saraiva
Mar 17, 2018·International Journal of Molecular Sciences·Nasir MehmoodIsabelle Gosselin
Oct 21, 2017·Applied Microbiology and Biotechnology·Irnayuli R SitepuKyria Boundy-Mills
Jan 14, 2018·Microbial Cell Factories·Scott BottomsJeff S Piotrowski
Mar 13, 2019·Biotechnology for Biofuels·Yusuke SasakiAindrila Mukhopadhyay
Mar 19, 2020·Applied Biochemistry and Biotechnology·Irnayuli SitepuKyria L Boundy-Mills
Nov 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jana FojtáškováPetr Pánek
Mar 24, 2018·Bioresource Technology·Shizeng WangQipeng Yuan
Nov 25, 2016·Current Opinion in Biotechnology·Julia I Martien, Daniel Amador-Noguez
Mar 20, 2019·Metabolic Engineering·Caleb WalkerCong T Trinh
May 12, 2021·The Science of the Total Environment·Chul-Woong ChoYeoung-Sang Yun
Nov 13, 2020·Journal of the American Chemical Society·Liang ZhengFu-Jian Xu

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Methods Mentioned

BETA
peptide assay
FACS
PCR

Software Mentioned

GCAT
Andromeda
GuavaExpress Pro
SigmaPlot
EdgeR
MaxQuant
MaxLFQ

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