PMID: 8611164Feb 1, 1996Paper

Mechanism of indole-3-acetic acid oxidation by plant peroxidases: anaerobic stopped-flow spectrophotometric studies on horseradish and tobacco peroxidases

The Biochemical Journal
I G GazaryanR N Thorneley

Abstract

Indole-3-acetic acid (IAA) is a powerful plant growth regulator. The oxidative decarboxylation of IAA by plant peroxidases is thought to be a major degradation reaction involved in controlling the in vivo level of IAA. Horseradish peroxidase isoenzyme C and an anionic tobacco peroxidase isolated from transgenic Nicotiana sylvestris have been used in experiments in vitro designed to determine the mechanism of IAA oxidation. In particular, the initial reduction of ferric to ferrous enzyme, a key step in previously proposed mechanisms, has been investigated by rapid-scan stopped-flow spectrophotometry under strictly anaerobic conditions and at defined oxygen concentrations. The data provide the first evidence for a ternary complex comprising peroxidase, IAA and oxygen that is kinetically competent both at the initiation stage and during the catalytic cycle of IAA oxidation. A general scheme describing the oxidative cycles of both anionic and cationic peroxidases is proposed that includes native ferric enzyme and compound II as kinetically competent intermediates. For anionic peroxidases, addition of hydrogen peroxide switches on the oxidative cycle thereby promoting IAA oxidation. 2-Methyl-IAA is not a substrate of the oxidase rea...Continue Reading

Citations

Mar 1, 2007·Pakistan Journal of Biological Sciences : PJBS·Houda BouaziziEzzeddine El Ferjani
Jan 3, 1997·The Journal of Biological Chemistry·J N Rodriguez-LopezR N Thorneley
May 15, 2002·Bioscience, Biotechnology, and Biochemistry·Tomonori KawanoFrédéric Lapeyrie
Dec 18, 2008·Journal of Experimental Botany·Claudia Cosio, Christophe Dunand
Dec 15, 2016·Chemistry, an Asian Journal·Hongping LiGuanghui Zhao
Apr 17, 2004·Physiologia Plantarum·Alexandra Keren-KeisermanIdit Ginzberg
Aug 5, 2005·The Journal of Biological Chemistry·Anna M VetranoJeffrey D Laskin
Feb 22, 2012·Applied Biochemistry and Biotechnology·Nuria González-RábadeJesús Agustín Badillo-Corona
Dec 14, 2002·The International Journal of Biochemistry & Cell Biology·Estrella Núñez-DelicadoAlvaro Sánchez-Ferrer
Apr 26, 2005·Bioorganic & Medicinal Chemistry·Ke-Qing Ling, Lawrence M Sayre
Jul 13, 2005·Journal of Plant Physiology·Soung Soo Kim, Dong Ju Lee
Aug 22, 2002·Journal of the American Chemical Society·Rheem A Totah, Robert P Hanzlik
Aug 15, 2009·Biochemical and Biophysical Research Communications·José SerranoManuel Portero-Otín

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.