PMID: 6172792Jan 1, 1982Paper

Mechanism of induction of prolactin synthesis in GH cells

Proceedings of the National Academy of Sciences of the United States of America
D K BiswasB A Brennessel

Abstract

Prolactin-specific RNA (RNA(PRL)) in total nuclear RNA and in cytoplasmic poly(A)(+)RNA isolated from GH (rat pituitary) cells was selectively hybridized to immobilized cloned cDNA(PRL). Agarose gel electrophoresis of the nuclear RNA(PRL) sequences eluted from the nitrocellulose filters revealed several RNA species of approximately 25-30, 18-19, and 12-13 S. Only the 12-13 S RNA species could be detected in the cytoplasmic poly(A)(+)RNA fraction. Comparative analysis of total nuclear RNA of control and thyrotropin-releasing hormone (thyroliberin)-treated cells by the reverse Southern blot technique demonstrated increased levels of all the nuclear RNA(PRL) species in hormone-treated cells. Nuclear and cytoplasmic RNA(PRL) sequences in control and treated cells were quantitated by molecular hybridization to cloned cDNA(PRL). The 2- to 3-fold stimulation of PRL production by thyrotropin-releasing hormone-treated GH(4)C(1) cells could be correlated to the corresponding increase of nuclear RNA(PRL) sequences. The hybrid strain, which produces 1/5th the amount of PRL that the parent GH(4)C(1) does, had 1/5th the amounts of nuclear RNA(PRL) sequences. Thyrotropin-releasing hormone affected neither prolactin production nor nuclear RNA(...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G K McMaster, G G Carmichael
Mar 30, 1979·Biochemical and Biophysical Research Communications·B A Brennessel, D K Biswas
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R P RicciardiB E Roberts
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J C AlwineG R Stark
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G A EvansM G Rosenfeld
Jul 27, 1976·Biochemistry·D K BiswasA H Tashjian
May 7, 1971·Biochemical and Biophysical Research Communications·A H TashjianD K Jensen
Jan 1, 1973·Annual Review of Biochemistry·W J RutterP W Morris
Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·D B Clewell, D R Helinski
Oct 1, 1980·The Journal of Cell Biology·B A Brennessel, D K Biswas

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