PMID: 2500444Jun 1, 1989Paper

Mechanism of inhibition of polypeptide chain initiation in calcium-depleted Ehrlich ascites tumor cells

The Journal of Cell Biology
R V KumarE C Henshaw

Abstract

Protein synthesis in Ehrlich ascites tumor cells is inhibited when cellular calcium is depleted by the addition of EGTA to the growth medium. This inhibition is at the level of polypeptide chain initiation as evidenced by a disaggregation of polyribosomes accompanied by a significant elevation in 80-S monomers. To identify direct effects of calcium on the protein synthesis apparatus we have developed a calcium-dependent, cell-free protein-synthesizing system from the Ehrlich cells by using 1,2-bis(O-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid (BAPTA), a recently developed chelator with a high (greater than 10(5)) selectivity for calcium (pKa = 6.97) over magnesium (pKa = 1.77). BAPTA inhibits protein synthesis by 70% at 1 mM and 90% at 2 mM. This effect was reversed by calcium but not by other cations tested. The levels of 43-S complexes (i.e., 40-S subunits containing bound methionyl-tRNAf.eIF-2.GTP) were significantly lower in the calcium-deprived incubations, indicating either inhibition of the rate of formation or decreased stability of 43-S complexes. Analysis of 43-S complexes on CsCl gradients showed that in BAPTA-treated lysates, 40-S subunits containing eIF-3, completely disappeared and the residual methionyl-tRNA-...Continue Reading

References

Dec 17, 1979·Biochimica Et Biophysica Acta·H Trachsel, T Staehelin
Jan 2, 1973·Biochimica Et Biophysica Acta·A CittadiniB Chance
Jan 1, 1980·Journal of Supramolecular Structure·A H LichtmanM A Lichtman
Jun 1, 1983·Archives of Biochemistry and Biophysics·S Ochoa
Apr 16, 1984·Biochimica Et Biophysica Acta·C D RichardsT R Hesketh
Apr 2, 1984·European Journal of Biochemistry·R Panniers, E C Henshaw
Sep 16, 1981·Biochemical and Biophysical Research Communications·F Homo, J Simon
Jan 1, 1980·European Journal of Biochemistry·A ThomasR Benne

❮ Previous
Next ❯

Citations

Jul 1, 1991·Developmental Biology·J P Kanki, J W Newport
Jan 1, 1991·Pharmacology & Therapeutics·S J Morley, G Thomas
Jan 1, 1994·Biochimie·S R Kimball, L S Jefferson
Oct 31, 2000·Journal of the Neurological Sciences·B C WhiteG S Krause
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A D ShortD L Gill
Dec 16, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D J DeGraciaG S Krause
Jan 30, 1991·European Journal of Biochemistry·R V KumarE C Henshaw
Dec 1, 1989·Current Opinion in Cell Biology·M J Clemens
Apr 15, 1991·Biochemical and Biophysical Research Communications·A E Brodie, D J Reed
Jun 11, 2005·The Journal of Biological Chemistry·Suzanne MiyamotoJohn W B Hershey
Apr 4, 2009·Physiological Reviews·Michael J Tisdale
Sep 9, 1992·Biochimica Et Biophysica Acta·P S Perkins, S J Pandol

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.