Mechanism of internal anal sphincter relaxation by CORM-1, authentic CO, and NANC nerve stimulation

American Journal of Physiology. Gastrointestinal and Liver Physiology
Satish RattanMárcio A F De Godoy

Abstract

The present studies compared the effects of CO-releasing molecule (CORM-1), authentic CO, and nonadrenergic noncholinergic (NANC) nerve stimulation in the internal anal sphincter (IAS). Functional in vitro experiments and Western blot studies were conducted in rat IAS smooth muscle. We examined the effects of CORM-1 (50-600 microM) and authentic CO (5-100 microM) and NANC nerve stimulation by electrical field stimulation (EFS; 0.5-20 Hz, 0.5-ms pulse, 12 V, 4-s train). The experiments were repeated after preincubation of the tissues with the neurotoxin TTX, the guanylate cyclase inhibitor 1H-(1,2,4)oxadiazolo-(4,3-a)quinoxalin-1-one (ODQ), the selective heme oxygenase (HO) inhibitor tin protoporphyrin IX (SnPP-IX), the nitric oxide synthase inhibitor N(omega)-nitro-L-arginine (L-NNA), and SnPP-IX + L-NNA. We also investigated the effects of the HO substrate hematin (100 microM). CORM-1, as well as CO, produced concentration-dependent IAS relaxation, whereas hematin had no effect. TTX abolished and L-NNA significantly blocked IAS relaxation by EFS without any effect on CORM-1 and CO. ODQ blocked IAS relaxation by CORM-1, authentic CO, and EFS. SnPP-IX had no significant effect on IAS relaxation by CORM-1, CO, or EFS. The presenc...Continue Reading

References

Dec 1, 1992·Gastroenterology·M E Stark, J H Szurszewski
Jan 1, 1992·The American Journal of Physiology·S Rattan, S Chakder
Jan 1, 1985·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·I VinogradS Nissan
Nov 1, 1988·The American Journal of Physiology·C Moummi, S Rattan
Jun 1, 1995·Annals of Medicine·R A Lefebvre
Oct 1, 1993·Gastroenterology·J M VanderwindenJ J Vanderhaeghen
Oct 1, 1993·The American Journal of Physiology·S Rattan, S Chakder
Jun 1, 1993·The American Journal of Physiology·G FarrugiaJ H Szurszewski
Jan 1, 1997·Annual Review of Pharmacology and Toxicology·M D Maines
Jul 16, 1997·European Journal of Pharmacology·T SekiH Demura
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·R ZakharyS H Snyder
Jan 22, 1998·The American Journal of Physiology·S ChakderS Rattan
Dec 22, 1999·The American Journal of Physiology·C ThorupM S Goligorsky
Dec 21, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·Y P FanS Rattan
Apr 12, 2001·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·S M MillerJ H Szurszewski
May 9, 2001·The Journal of Clinical Investigation·S P Cary, M A Marletta
Feb 9, 2002·Circulation Research·Roberto MotterliniColin J Green
Feb 14, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ya-Ping FanSatish Rattan
Mar 26, 2002·The Journal of Urology·Annette SchroderKarl-Erik Andersson
Jul 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Weibiao CaoPiero Biancani
Jul 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Gianrico FarrugiaJoseph H Szurszewski
Oct 7, 2003·Annual Review of Neuroscience·Darren Boehning, Solomon H Snyder
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Crystal C WatkinsSolomon H Snyder

❮ Previous
Next ❯

Citations

Nov 8, 2005·Naunyn-Schmiedeberg's Archives of Pharmacology·Maram G KatoueMabayoje A Oriowo
Jun 20, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Ole De BackerRomain A Lefebvre
Sep 4, 2004·Antioxidants & Redox Signaling·Cesare Mancuso
Jul 6, 2007·European Journal of Pharmacology·Ole De Backer, Romain A Lefebvre
Apr 20, 2005·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·S Rattan
Jul 2, 2009·Autonomic & Autacoid Pharmacology·K Mills, R Chess-Williams
Nov 1, 2005·Expert Opinion on Investigational Drugs·Roberto MotterliniRoberta Foresti
Apr 23, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Akiko TerauchiHiroshi Mashimo
Nov 24, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Roberto MotterliniColin J Green
Jun 10, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Chirag A Patel, Satish Rattan
Jun 3, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Arjun Singh, Satish Rattan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.