PMID: 1390925Sep 23, 1992

Mechanism of ligand-protein interaction in plant seed thiamin-binding proteins. Probing the binding site of protein isolated from buckwheat seeds with a series of thiamin-related compounds

Biochimica Et Biophysica Acta
M Rapała-Kozik, A Kozik

Abstract

Affinities of 14 thiamin derivatives or antagonists to a thiamin-binding protein isolated from buckwheat seeds were determined. A competitive displacement of radiolabeled thiamin by unlabeled ligand was analysed by a computerized model-fitting procedure. The dissociation constant of the thiamin-protein complex was 0.93 microM. Most modifications in ligand chemical structure weakened the ligand-protein interaction. A model of the thiamin-binding site is suggested. The hydroxyethyl-chain of thiamin while protein-bound appears to be excluded from the binding region. A positively charged quaternary nitrogen atom of the thiazolium ring probably interacts with some negative group(s) of protein. The rest of the thiazolium ring as well as the amino group of the pyrimidine fragment serve as additional anchors. The three structural features of the thiamin molecule accounting for binding contribute equally to overall binding energy by about 11-12 kJ/mol.

References

Jan 1, 1977·Journal of Supramolecular Structure·G B HendersonF M Huennekens
Oct 1, 1988·Archives of Biochemistry and Biophysics·H NishimuraA Iwashima
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Oct 1, 1980·Archives of Biochemistry and Biophysics·J D Choi, D B McCormick
Jul 26, 2011·International Journal of Systematic and Evolutionary Microbiology·Byoung-Jun KimBum-Joon Kim

Citations

Mar 1, 1994·Journal of Biochemical and Biophysical Methods·A Kozik
Mar 31, 2004·Plant Physiology and Biochemistry : PPB·Anna GołdaM Rapała-Kozik
Apr 6, 2016·Journal of Medicinal Chemistry·Christiane EhrtOliver Koch
Aug 1, 1995·Biological Reviews of the Cambridge Philosophical Society·P R Shewry
Dec 12, 2012·PloS One·Bruno Moreira, Juli G Pausas
Jan 6, 2015·The Journal of Ecology·Torbjørn R PaulsenMichelle Leishman

Related Concepts

Thiamine-binding protein
Carrier Proteins
Triticale
Ligands
Thiamine Mononitrate

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