Mechanism of lysozyme adsorption onto gold surface determined by quartz crystal microbalance and surface plasmon resonance.

Bioelectrochemistry
P KomorekBarbara Jachimska

Abstract

In this study, the physicochemical characterization of lysozyme adsorbed on gold was investigated. Through the use of MP-SPR it was possible to establish that the orientation of molecules changes from side-on to between or end-on with increasing surface coverage. The data confirms that the process of adsorption is driven primarily by electrostatic interactions but also by hydrophobic forces. MP-SPR data was compared with the Random Sequential Adsorption model for a molecule with an ellipsoidal shape. Contact angle measurements showed that higher surface coverage also translates in more hydrophilic properties of obtained lysozyme layer. Comparison of CD and PM-IRRAS spectra in solution and adsorbed state respectively showed changes in the secondary structures of lysozyme. These changes are dependent on pH, but fundamentally they go in the direction of the increase of β-turn/random content with a simultaneous decrease in β-sheet fraction, which suggests that aggregation is not occurring. The combination of MP-SPR and QCM-D measurements allowed the estimation of the number of water molecules associated with the lysozymes films. It has been observed that hydration decreases from 70% in pH = 4 to 30% in pH = 11. This data indicates ...Continue Reading

References

Jan 20, 1965·Journal of the American Chemical Society·G HOLZWARTH, P DOTY
Nov 16, 2004·Biophysical Journal·Ananthakrishnan Sethuraman, Georges Belfort
Feb 23, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Mamdouh E AbdelsalamJeremy Baumberg
Dec 1, 2006·Colloids and Surfaces. B, Biointerfaces·Andreas W SonessonUlla M Elofsson
Feb 24, 2007·Colloids and Surfaces. B, Biointerfaces·Susan M DalyRobert D Tilton
Aug 19, 2007·Biochimica Et Biophysica Acta·Andreas Barth
Feb 16, 2008·Biopolymers·Anna Marie C Marcelino, Lila M Gierasch
Jan 23, 2009·Prion·Roma N Rambaran, Louise C Serpell
Apr 2, 2009·Biochimica Et Biophysica Acta·Satish KumarRajaram Swaminathan
Feb 2, 2010·Colloids and Surfaces. B, Biointerfaces·Maria LundinMark W Rutland
Feb 8, 2011·Advances in Colloid and Interface Science·Michael RabeStefan Seeger
Oct 22, 2011·PloS One·Bradley MooresZoya Leonenko
Jul 13, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·B JachimskaA Pajor-Świerzy
Jun 20, 2014·Chemical Communications : Chem Comm·Daewha HongWoo Kyung Cho
Dec 19, 2014·Expert Opinion on Drug Delivery·Darrell Sleep
Feb 25, 2015·Nanomedicine·Dominic DocterRoland H Stauber
Sep 1, 2015·Physical Chemistry Chemical Physics : PCCP·Karina Kubiak-OssowskaPaul A Mulheran
Oct 16, 2015·Advanced Healthcare Materials·Yihua LooAnna Mitraki
Jan 27, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Qiang Peng, Huiling Mu
Aug 1, 2016·The Journal of Chemical Physics·Michał CieślaRobert M Ziff
Feb 25, 2017·Physical Chemistry Chemical Physics : PCCP·B A RussellY Chen
Feb 28, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Gustavo GuzmanMukerrem Cakmak
Mar 30, 2017·The Journal of Physical Chemistry. B·Karina Kubiak-OssowskaPaul A Mulheran
Dec 10, 2017·Colloids and Surfaces. B, Biointerfaces·F Romero-GavilanI Goñi
Oct 6, 2018·International Journal of Biological Macromolecules·Jarosław WawerJoanna Krakowiak
Dec 28, 2018·Bioelectrochemistry·S ŚwiątekB Jachimska

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.