Mechanism of macromolecule concentration in collecting lymphatics in rat mesentery

Microvascular Research
T TakahashiA Kamiya

Abstract

The mechanism by which macromolecular contents are concentrated during lymph transport was investigated in the collecting lymphatics of the mesentery of the rat. To examine changes in the concentration of lymph macromolecular tracer (fluorescein isothiocyanate-tagged dextran, MW 148,900) in the rhythmically contractile lymphatics, we quantified the fluorescence intensity of the lymph tracer using an intravital microscopy system with single slit-laser (20 microns in thickness) epi-illumination, regardless of the vasomotion of the lymph vessel. The results for the fluorescence intensity measurement at different sites at distances of about 300-1000 microns along unbranched collecting lymphatics revealed that the concentration of the lymph macromolecular contents was significantly increased during their passage from upstream to downstream (P < 0.05). The fluorescence intensity of lymph tracer in the occluded segment of collecting lymphatics around 1000 microns in length was enhanced during the contraction phase and reduced during the relaxation phase. These results suggest that the filtration of macromolecule-free fluid from the lymph across the lymphatic wall during its contraction plays an important role in the lymph concentratio...Continue Reading

References

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Citations

Sep 30, 2005·Lymphatic Research and Biology·Risuke MizunoToshio Ohhashi
Nov 18, 2009·The Journal of Physiology·Joshua P Scallan, Virginia H Huxley
Mar 2, 2016·Annual Review of Biomedical Engineering·Susan N ThomasErin E Edwards
Jun 21, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Nobuyuki OnoToshio Ohhashi
Jan 1, 2012·Annals of Vascular Diseases·Fumitaka IkomiToshio Ohhashi

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