Mechanism of methaemoglobin breakdown by the lysine-specific gingipain of the periodontal pathogen Porphyromonas gingivalis.

Biological Chemistry
J W SmalleyJan Potempa

Abstract

Abstract The R- and K-gingipain proteases of Porphyromonas gingivalis are involved in proteolysis of haemoglobin from which the defensive dimeric haem pigment is formed. Whilst oxyhaemoglobin is refractory towards K-gingipain, methaemoglobin is rapidly degraded. Ligation of methaemoglobin with N3-, which effectively blocks haem dissociation from the protein, prevented haemoglobin breakdown. Haem-free globin was rapidly degraded by K-gingipain. These data emphasise the need for haemoglobin oxidation which encourages haem dissociation and makes the haem-free globin susceptible to proteolytic attack.

References

Feb 1, 1974·European Journal of Biochemistry·G H BeavenW B Gratzer
Jan 1, 1981·Methods in Enzymology·F AscoliE Antonini
Sep 3, 1996·Biochemistry·M S Hargrove, J S Olson
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·R J Lamont, H F Jenkinson
May 12, 2000·Journal of Periodontology·T HaniokaS Shizukuishi
Apr 1, 1965·Journal of Molecular Biology·M J CRUMPTON, A POLSON
Feb 12, 2004·Oral Microbiology and Immunology·P I Diaz, A H Rogers

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Citations

Oct 14, 2010·The Journal of Biological Chemistry·Jin-Long GaoNeil Hunter
Sep 17, 2009·Journal of Innate Immunity·Jan Potempa, Robert N Pike
May 9, 2009·PLoS Pathogens·Halina WójtowiczF Xavier Gomis-Rüth
May 13, 2015·Journal of Bacteriology·Austen TerwilligerAnthony Maresso
May 27, 2016·Microbiology Spectrum·Jessica R SheldonDavid E Heinrichs

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